Literature DB >> 22200928

Highly sensitive detection of microRNA by chemiluminescence based on enzymatic polymerization.

Changbei Ma1, Edward S Yeung, Shengda Qi, Rui Han.   

Abstract

We have developed a new methodology for miRNA assay using chemiluminescence imaging by poly(U) polymerase catalyzed miRNA polymerization. This method is very sensitive with a 50 fM limit of detection, which is comparable to or better than current assay methods. Multiplex detection for miRNA can be easily realized by introducing different capture probes onto the biosensor array, which will make it highly versatile for various research purposes.

Mesh:

Substances:

Year:  2011        PMID: 22200928     DOI: 10.1007/s00216-011-5653-4

Source DB:  PubMed          Journal:  Anal Bioanal Chem        ISSN: 1618-2642            Impact factor:   4.142


  3 in total

Review 1.  Types and Applications of Nicking Enzyme-Combined Isothermal Amplification.

Authors:  Siyu Cao; Xiaochen Tang; Tianshu Chen; Guifang Chen
Journal:  Int J Mol Sci       Date:  2022-04-21       Impact factor: 6.208

Review 2.  Therapeutic evaluation of microRNAs by molecular imaging.

Authors:  Thillai V Sekar; Ramkumar Kunga Mohanram; Kira Foygel; Ramasamy Paulmurugan
Journal:  Theranostics       Date:  2013-12-06       Impact factor: 11.556

3.  An Exonuclease I-Based Quencher-Free Fluorescent Method Using DNA Hairpin Probes for Rapid Detection of MicroRNA.

Authors:  Changbei Ma; Haisheng Liu; Kefeng Wu; Mingjian Chen; Liyang Zheng; Jun Wang
Journal:  Sensors (Basel)       Date:  2017-04-03       Impact factor: 3.576

  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.