OBJECTIVES: The molecular mechanism of postoperative cognitive dysfunction is largely unknown. Isoflurane has been shown to promote Alzheimer's disease neuropathogenesis. We set out to determine whether the effect of isoflurane on spatial memory is associated with amyloid-beta (A-beta) levels and tau phosphorylation in aged rats. METHODS: Eighteen-month-old male Sprague-Dawley rats were randomly assigned as anesthesia group (n = 31, received 1.4% isoflurane for 2 hours and had behavioral testing), training group (n = 20, received no anesthesia but had behavioral testing), and control group (n = 10, received no anesthesia and had no behavioral testing). Spatial memory was measured before and 2 days after the anesthesia by the Morris water maze. We divided the anesthesia group into an isoflurane-induced severe memory impairment group (SIG, n = 6) and a no severe memory impairment group (NSIG, n = 25), according to whether the escape latency was more than 1.96 stand deviation of that from the training group. Levels of A-beta and tau in the hippocampus were determined by enzyme-linked immunosorbent assay and quantitative western blot at the end of behavioral testing. RESULTS: We found that isoflurane increased the escape latency in the SIG as compared to that in the training group and NSIG without affecting swimming speed. However, there were no differences in the levels of A-beta and tau among SIG, NSIG, training, and control groups. CONCLUSIONS: Isoflurane may induce spatial memory impairment through non-A-beta or tau neuropathogenesis mechanisms in aged rats.
OBJECTIVES: The molecular mechanism of postoperative cognitive dysfunction is largely unknown. Isoflurane has been shown to promote Alzheimer's disease neuropathogenesis. We set out to determine whether the effect of isoflurane on spatial memory is associated with amyloid-beta (A-beta) levels and tau phosphorylation in aged rats. METHODS: Eighteen-month-old male Sprague-Dawley rats were randomly assigned as anesthesia group (n = 31, received 1.4% isoflurane for 2 hours and had behavioral testing), training group (n = 20, received no anesthesia but had behavioral testing), and control group (n = 10, received no anesthesia and had no behavioral testing). Spatial memory was measured before and 2 days after the anesthesia by the Morris water maze. We divided the anesthesia group into an isoflurane-induced severe memory impairment group (SIG, n = 6) and a no severe memory impairment group (NSIG, n = 25), according to whether the escape latency was more than 1.96 stand deviation of that from the training group. Levels of A-beta and tau in the hippocampus were determined by enzyme-linked immunosorbent assay and quantitative western blot at the end of behavioral testing. RESULTS: We found that isoflurane increased the escape latency in the SIG as compared to that in the training group and NSIG without affecting swimming speed. However, there were no differences in the levels of A-beta and tau among SIG, NSIG, training, and control groups. CONCLUSIONS:Isoflurane may induce spatial memory impairment through non-A-beta or tau neuropathogenesis mechanisms in aged rats.
Authors: Juan Perucho; Isabel Rubio; Maria J Casarejos; Ana Gomez; Jose A Rodriguez-Navarro; Rosa M Solano; Justo Garcia De Yébenes; Maria A Mena Journal: J Alzheimers Dis Date: 2010 Impact factor: 4.472
Authors: Roderic G Eckenhoff; Jonas S Johansson; Huafeng Wei; Anna Carnini; Baobin Kang; Wenlin Wei; Ravindernath Pidikiti; Jason M Keller; Maryellen F Eckenhoff Journal: Anesthesiology Date: 2004-09 Impact factor: 7.892
Authors: Charles H Brown; Charles Edwards; Charles Lin; Emily Ledford Jones; Lisa R Yanek; Melody Esmaili; Yara Gorashi; Richard Skelton; Daniel Kaganov; Ryan Curto; Noah L Lessing; Stephanie Cha; Elizabeth Colantuoni; Karin Neufeld; Frederick Sieber; Clayton L Dean; Charles W Hogue Journal: Anesthesiology Date: 2021-12-01 Impact factor: 7.892