Literature DB >> 22183674

Random insertional mutagenesis in fungal genomes to identify virulence factors.

Parthasarathy Santhanam1.   

Abstract

Agrobacterium tumefaciens-mediated transformation (ATMT) has become an important tool for functional genomics in fungi. ATMT-based approaches such as random insertional mutagenesis and targeted knockout are widely used for gene functional analysis in plant-pathogen interactions. Here, we describe a protocol for the identification of pathogenicity and virulence genes through random insertional mutagenesis using the fungal wilt pathogen Verticillium dahliae as an example for the protocol.

Entities:  

Mesh：

Substances：

Year:  2012        PMID： 22183674     DOI： 10.1007/978-1-61779-501-5_31

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  3 in total

1.  Repetitive Elements Contribute to the Diversity and Evolution of Centromeres in the Fungal Genus Verticillium.

Authors:  Michael F Seidl; H Martin Kramer; David E Cook; Gabriel L Fiorin; Grardy C M van den Berg; Luigi Faino; Bart P H J Thomma
Journal:  mBio       Date:  2020-09-08       Impact factor: 7.867

2.  Three putative DNA methyltransferases of Verticillium dahliae differentially contribute to DNA methylation that is dispensable for growth, development and virulence.

Authors:  H Martin Kramer; David E Cook; Grardy C M van den Berg; Michael F Seidl; Bart P H J Thomma
Journal:  Epigenetics Chromatin       Date:  2021-05-03       Impact factor: 4.954

3.  Local Rather than Global H3K27me3 Dynamics Are Associated with Differential Gene Expression in Verticillium dahliae.

Authors:  H Martin Kramer; Michael F Seidl; Bart P H J Thomma; David E Cook
Journal:  mBio       Date:  2022-02-08       Impact factor: 7.867
  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.