Literature DB >> 22183664

Polyethylene glycol (PEG)-mediated transformation in filamentous fungal pathogens.

Zhaohui Liu1, Timothy L Friesen.   

Abstract

Genetic transformation is an essential tool for the modern study of gene function and the genetic improvement of an organism. The genetic transformation of many fungal species is well established and can be carried out by utilizing different transformation methods including electroporation, Agrobacterium, biolistics, or polyethylene glycol (PEG)-mediated transformation. Due to its technical simplicity and common equipment requirements, PEG-mediated transformation is still the most commonly used method for genetic transformation in filamentous fungi. Here, we describe a PEG-based protocol developed for genetic transformation of Stagonospora nodorum, a fungal pathogen of wheat. This protocol is directly applicable to other fungi especially those in the Dothideomycete class of fungi.

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Year:  2012        PMID： 22183664     DOI： 10.1007/978-1-61779-501-5_21

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  26 in total

1.  Agrobacterium-mediated transformation of the ascomycete mushroom Morchella importuna using polyubiquitin and glyceraldehyde-3-phosphate dehydrogenase promoter-based binary vectors.

Authors:  Shouyun Lv; Xin Chen; Chunye Mou; Shenghong Dai; Yinbing Bian; Heng Kang
Journal:  World J Microbiol Biotechnol       Date:  2018-09-14       Impact factor: 3.312

2.  Involvement of threonine deaminase FgIlv1 in isoleucine biosynthesis and full virulence in Fusarium graminearum.

Authors:  Xin Liu; Jianhong Xu; Jian Wang; Fang Ji; Xianchao Yin; Jianrong Shi
Journal:  Curr Genet       Date:  2014-08-17       Impact factor: 3.886

3.  The potential protein kinase A (Pka) phosphorylation site is required for the function of FgSge1 in Fusarium graminearum.

Authors:  Fang-Wei Yu; Xiao-Ping Zhang; Meng-Hao Yu; Yan-Ni Yin; Zhong-Hua Ma
Journal:  World J Microbiol Biotechnol       Date:  2015-07-01       Impact factor: 3.312

4.  A new and efficient approach for construction of uridine/uracil auxotrophic mutants in the filamentous fungus Aspergillus oryzae using Agrobacterium tumefaciens-mediated transformation.

Authors:  Khuyen Thi Nguyen; Quynh Ngoc Ho; Loc Thi Binh Xuan Do; Linh Thi Dam Mai; Duc-Ngoc Pham; Huyen Thi Thanh Tran; Diep Hong Le; Huy Quang Nguyen; Van-Tuan Tran
Journal:  World J Microbiol Biotechnol       Date:  2017-05-02       Impact factor: 3.312

5.  The construction and use of versatile binary vectors carrying pyrG auxotrophic marker and fluorescent reporter genes for Agrobacterium-mediated transformation of Aspergillus oryzae.

Authors:  Khuyen Thi Nguyen; Quynh Ngoc Ho; Thu Ha Pham; Tuan-Nghia Phan; Van-Tuan Tran
Journal:  World J Microbiol Biotechnol       Date:  2016-11-01       Impact factor: 3.312

6.  Gene Disruption in Scedosporium aurantiacum: Proof of Concept with the Disruption of SODC Gene Encoding a Cytosolic Cu,Zn-Superoxide Dismutase.

Authors:  Victoire Pateau; Bienvenue Razafimandimby; Patrick Vandeputte; Christopher R Thornton; Thomas Guillemette; Jean-Philippe Bouchara; Sandrine Giraud
Journal:  Mycopathologia       Date:  2017-10-11       Impact factor: 2.574

7.  The tubulin cofactor A is involved in hyphal growth, conidiation and cold sensitivity in Fusarium asiaticum.

Authors:  Xiaoping Zhang; Xiang Chen; Jinhua Jiang; Menghao Yu; Yanni Yin; Zhonghua Ma
Journal:  BMC Microbiol       Date:  2015-02-18       Impact factor: 3.605

8.  The Parastagonospora nodorum necrotrophic effector SnTox5 targets the wheat gene Snn5 and facilitates entry into the leaf mesophyll.

Authors:  Gayan K Kariyawasam; Jonathan K Richards; Nathan A Wyatt; Katherine L D Running; Steven S Xu; Zhaohui Liu; Pawel Borowicz; Justin D Faris; Timothy L Friesen
Journal:  New Phytol       Date:  2021-08-03       Impact factor: 10.323

9.  BZcon1, a SANT/Myb-type gene involved in the conidiation of Cochliobolus carbonum.

Authors:  Jun-xiang Zhang; Yi-xin Wu; Honhing Ho; Hao Zhang; Peng-fei He; Yue-qiu He
Journal:  G3 (Bethesda)       Date:  2014-06-03       Impact factor: 3.154

10.  Acetohydroxyacid synthase FgIlv2 and FgIlv6 are involved in BCAA biosynthesis, mycelial and conidial morphogenesis, and full virulence in Fusarium graminearum.

Authors:  Xin Liu; Qi Han; Jianhong Xu; Jian Wang; Jianrong Shi
Journal:  Sci Rep       Date:  2015-11-10       Impact factor: 4.379
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