Literature DB >> 22183603

Analysis of changes in nucleosome conformation using fluorescence resonance energy transfer.

Tina Shahian1, Geeta J Narlikar.   

Abstract

ATP-dependent nucleosome-remodeling motors use the energy of ATP to alter the accessibility of the underlying DNA. Understanding how these motors alter nucleosome structure can be aided by following changes in histone-DNA contacts in real time. Here, we describe a fluorescence resonance energy transfer-based approach that enables visualization of such changes.

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Year:  2012        PMID: 22183603     DOI: 10.1007/978-1-61779-477-3_20

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN: 1064-3745


  10 in total

1.  A nucleotide-driven switch regulates flanking DNA length sensing by a dimeric chromatin remodeler.

Authors:  John D Leonard; Geeta J Narlikar
Journal:  Mol Cell       Date:  2015-02-12       Impact factor: 17.970

2.  HDAC8 substrate selectivity is determined by long- and short-range interactions leading to enhanced reactivity for full-length histone substrates compared with peptides.

Authors:  Carol Ann Castañeda; Noah A Wolfson; Katherine R Leng; Yin-Ming Kuo; Andrew J Andrews; Carol A Fierke
Journal:  J Biol Chem       Date:  2017-11-06       Impact factor: 5.157

3.  Monitoring conformational dynamics with single-molecule fluorescence energy transfer: applications in nucleosome remodeling.

Authors:  Sebastian Deindl; Xiaowei Zhuang
Journal:  Methods Enzymol       Date:  2012       Impact factor: 1.600

4.  Reconstitution and Purification of Nucleosomes with Recombinant Histones and Purified DNA.

Authors:  Ilana M Nodelman; Ashok Patel; Robert F Levendosky; Gregory D Bowman
Journal:  Curr Protoc Mol Biol       Date:  2020-12

5.  Decoupling nucleosome recognition from DNA binding dramatically alters the properties of the Chd1 chromatin remodeler.

Authors:  Ashok Patel; Srinivas Chakravarthy; Seamus Morrone; Ilana M Nodelman; Jeffrey N McKnight; Gregory D Bowman
Journal:  Nucleic Acids Res       Date:  2012-12-28       Impact factor: 16.971

6.  The Chd1 chromatin remodeler shifts hexasomes unidirectionally.

Authors:  Robert F Levendosky; Anton Sabantsev; Sebastian Deindl; Gregory D Bowman
Journal:  Elife       Date:  2016-12-29       Impact factor: 8.140

7.  Diverse nucleosome Site-Selectivity among histone deacetylase complexes.

Authors:  Zhipeng A Wang; Christopher J Millard; Chia-Liang Lin; Jennifer E Gurnett; Mingxuan Wu; Kwangwoon Lee; Louise Fairall; John Wr Schwabe; Philip A Cole
Journal:  Elife       Date:  2020-06-05       Impact factor: 8.140

8.  Ion counting demonstrates a high electrostatic field generated by the nucleosome.

Authors:  Magdalena Gebala; Stephanie L Johnson; Geeta J Narlikar; Dan Herschlag
Journal:  Elife       Date:  2019-06-11       Impact factor: 8.140

9.  Direct observation of coordinated DNA movements on the nucleosome during chromatin remodelling.

Authors:  Anton Sabantsev; Robert F Levendosky; Xiaowei Zhuang; Gregory D Bowman; Sebastian Deindl
Journal:  Nat Commun       Date:  2019-04-12       Impact factor: 14.919

10.  Spatiotemporally controlled generation of NTPs for single-molecule studies.

Authors:  Anton Sabantsev; Guanzhong Mao; Javier Aguirre Rivera; Mikhail Panfilov; Anatolii Arseniev; Oanh Ho; Mikhail Khodorkovskiy; Sebastian Deindl
Journal:  Nat Chem Biol       Date:  2022-09-21       Impact factor: 16.174

  10 in total

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