| Literature DB >> 22183588 |
Juan Jose Infante1, G Lynn Law, Elton T Young.
Abstract
The nucleosome-scanning assay (NuSA) couples isolation of mononucleosomal DNA after micrococcal nuclease (MNase) digestion with quantitative real-time PCR (qPCR) to map nucleosome positions in chromatin. It is a relatively simple, rapid procedure that can produce a high-resolution map of nucleosome location and occupancy and thus is suitable for analyzing individual promoters in great detail. The analysis can also quantify the protection of DNA sequences due to interaction with proteins other than nucleosomes and show how this protection varies when conditions change. When coupled with chromatin immunoprecipitation (ChIP), NuSA can identify histone variants and modifications associated with specific nucleosomes.Mesh：
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Year: 2012 PMID： 22183588 DOI： 10.1007/978-1-61779-477-3_5
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745