BACKGROUND: Bacterin-based canine Leptospira vaccines could present a challenge for the use of whole blood real-time polymerase chain reaction (PCR) as a diagnostic tool. Recent vaccination could induce positive results if the targeted DNA fragment is present within the vaccine and in the blood of the recently vaccinated dog. OBJECTIVES: The objective of this study was to assess whether 2 available 4-serovar vaccines induce a positive real-time PCR reaction in the blood of healthy recently vaccinated dogs. ANIMALS: Twenty healthy dogs. METHODS: This was a prospective study. Dogs were assigned to 1 of 2 vaccine groups. Both vaccines were culture-based and include Leptospira interrogans serovars Pomona, Canicola, and Icterohaemorrhagiae and Leptospira kirschneri serovar Grippotyphosa. Whole blood for real-time PCR and serum for the microscopic agglutination test (MAT) were collected prior to and 3 and 7 days after vaccination and weekly thereafter for 8 weeks. Two real-time PCR tests targeting 2 different genes were performed independently in a blinded fashion. RESULTS: Both Leptospira vaccines produced positive real-time PCR reactions when assayed undiluted or diluted 1 : 100 in canine blood. However, blood samples drawn from all dogs at all time points after vaccination were negative on PCR. All dogs developed MAT titers. CONCLUSIONS AND CLINICAL IMPORTANCE: Recent vaccination with 2 commercially available vaccines does not interfere with the use of real-time PCR for the identification of acute Leptospira infection in dogs.
BACKGROUND: Bacterin-based canineLeptospira vaccines could present a challenge for the use of whole blood real-time polymerase chain reaction (PCR) as a diagnostic tool. Recent vaccination could induce positive results if the targeted DNA fragment is present within the vaccine and in the blood of the recently vaccinated dog. OBJECTIVES: The objective of this study was to assess whether 2 available 4-serovar vaccines induce a positive real-time PCR reaction in the blood of healthy recently vaccinated dogs. ANIMALS: Twenty healthy dogs. METHODS: This was a prospective study. Dogs were assigned to 1 of 2 vaccine groups. Both vaccines were culture-based and include Leptospira interrogans serovars Pomona, Canicola, and Icterohaemorrhagiae and Leptospira kirschneri serovar Grippotyphosa. Whole blood for real-time PCR and serum for the microscopic agglutination test (MAT) were collected prior to and 3 and 7 days after vaccination and weekly thereafter for 8 weeks. Two real-time PCR tests targeting 2 different genes were performed independently in a blinded fashion. RESULTS: Both Leptospira vaccines produced positive real-time PCR reactions when assayed undiluted or diluted 1 : 100 in canine blood. However, blood samples drawn from all dogs at all time points after vaccination were negative on PCR. All dogs developed MAT titers. CONCLUSIONS AND CLINICAL IMPORTANCE: Recent vaccination with 2 commercially available vaccines does not interfere with the use of real-time PCR for the identification of acute Leptospira infection in dogs.
Authors: A Barthélemy; M Magnin; C Pouzot-Nevoret; J-M Bonnet-Garin; M Hugonnard; I Goy-Thollot Journal: J Vet Intern Med Date: 2016-12-02 Impact factor: 3.333
Authors: Andrea M Spiri; Sabrina Rodriguez-Campos; José M Matos; Tony M Glaus; Barbara Riond; Claudia E Reusch; Regina Hofmann-Lehmann; Barbara Willi Journal: BMC Vet Res Date: 2017-05-25 Impact factor: 2.741
Authors: Katie E McCallum; Fernando Constantino-Casas; John M Cullen; James H Warland; Harry Swales; Niamh Linghley; Andre J Kortum; Alex J Sterritt; Tristan Cogan; Penny J Watson Journal: J Vet Intern Med Date: 2018-11-29 Impact factor: 3.333
Authors: L E R Martin; K T Wiggans; S A Wennogle; K Curtis; R Chandrashekar; M R Lappin Journal: J Vet Intern Med Date: 2014-03-05 Impact factor: 3.175