| Literature DB >> 22178963 |
Natesan A L Flower1, Balusamy Brabu, Murugan Revathy, Chandrasekaran Gopalakrishnan, Savarimuthu V K Raja, Siva S Murugan, Tirukalikundram S Kumaravel.
Abstract
Nano-silver (Nano-Ag) particles were synthesized and then characterized using transmission electron microscopy (TEM) and X-ray diffractometry. TEM showed that Nano-Ag were spherical in shape and their size ranged from 40 to 60nm. X-ray diffractometry indicated that the sample was crystalline and had a face centered cubic structure of pure silver. Genotoxicity of this Nano-Ag was evaluated in human peripheral blood cells using the alkaline comet assay. Results indicated that Nano-Ag (50 and 100μg/mL) caused DNA damage following a 3h treatment. Subsequently, a short treatment of 5min also showed DNA damage. In conclusion, we have shown that the synthesized Nano-Ag induced DNA damage in human peripheral blood cells as detected by the alkaline comet assay. Results further indicated that treatment of cells with Nano-Ag in the presence of hydrogen peroxide did not induce any DNA damage.Entities:
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Year: 2011 PMID： 22178963 DOI： 10.1016/j.mrgentox.2011.12.003
Source DB: PubMed Journal: Mutat Res ISSN： 0027-5107 Impact factor: 2.433