Literature DB >> 22178063

Ligand-induced clustering of EGF receptors: a quantitative study by fluorescence image moment analysis.

Mikhail Sergeev1, Jody L Swift, Antoine G Godin, Paul W Wiseman.   

Abstract

Fluorescence microscopy is widely used in the life sciences, but largely for qualitative imaging. Here we apply a bioanalytical technique, fluorescence image moment analysis, to demonstrate how the distribution of the fluorescent molecules can be measured directly from confocal microscopy images. We measured the oligomerization state of EGF-eGPF receptors expressed in CHO-K1 cells in situ.
Copyright © 2011 Elsevier B.V. All rights reserved.

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Year:  2011        PMID: 22178063     DOI: 10.1016/j.bpc.2011.11.003

Source DB:  PubMed          Journal:  Biophys Chem        ISSN: 0301-4622            Impact factor:   2.352


  6 in total

1.  Spatial Intensity Distribution Analysis Reveals Abnormal Oligomerization of Proteins in Single Cells.

Authors:  Antoine G Godin; Benjamin Rappaz; Laurent Potvin-Trottier; Timothy E Kennedy; Yves De Koninck; Paul W Wiseman
Journal:  Biophys J       Date:  2015-08-18       Impact factor: 4.033

Review 2.  Scanning number and brightness yields absolute protein concentrations in live cells: a crucial parameter controlling functional bio-molecular interaction networks.

Authors:  Christina Papini; Catherine A Royer
Journal:  Biophys Rev       Date:  2018-01-30

3.  Studying KcsA Channel Clustering Using Single Channel Voltage-Clamp Fluorescence Imaging.

Authors:  Hugo McGuire; Rikard Blunck
Journal:  Front Physiol       Date:  2022-06-03       Impact factor: 4.755

4.  Plasma Membrane Organization of Epidermal Growth Factor Receptor in Resting and Ligand-Bound States.

Authors:  Nirmalya Bag; Shuangru Huang; Thorsten Wohland
Journal:  Biophys J       Date:  2015-11-03       Impact factor: 4.033

5.  Determination of membrane protein transporter oligomerization in native tissue using spatial fluorescence intensity fluctuation analysis.

Authors:  Mikhail Sergeev; Antoine G Godin; Liyo Kao; Natalia Abuladze; Paul W Wiseman; Ira Kurtz
Journal:  PLoS One       Date:  2012-04-27       Impact factor: 3.240

6.  Time-resolved multimodal analysis of Src Homology 2 (SH2) domain binding in signaling by receptor tyrosine kinases.

Authors:  Joshua A Jadwin; Dongmyung Oh; Timothy G Curran; Mari Ogiue-Ikeda; Lin Jia; Forest M White; Kazuya Machida; Ji Yu; Bruce J Mayer
Journal:  Elife       Date:  2016-04-12       Impact factor: 8.140

  6 in total

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