| Literature DB >> 22177907 |
Tomonori Muro1, Junko Fujihara, Shinji Imamura, Hiroaki Nakamura, Kaori Kimura-Kataoka, Tomoko Toga, Reiko Iida, Toshihiro Yasuda, Haruo Takeshita.
Abstract
ABO grouping of biological specimens is informative for identifying victims and narrowing down suspects. In Japan and elsewhere, ABO grouping as well as DNA profiling plays an essential role in crime investigations. In the present study, we developed a new method for ABO genotyping using allele-specific primers and real-time PCR. The method allows for the detection of three single nucleotide polymorphisms (SNPs) at nucleotide positions 261, 796, and 803 in the ABO gene and the determination of six major ABO genotypes. This method required less than 2 h for accurate ABO genotyping using 2.0 ng of DNA. This method could be applicable for rapid and simple screening of forensic samples.Entities:
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Year: 2011 PMID： 22177907 DOI： 10.1016/j.legalmed.2011.10.002
Source DB: PubMed Journal: Leg Med (Tokyo) ISSN： 1344-6223 Impact factor: 1.376