| Literature DB >> 22171566 |
Abstract
In this paper we report stimulated emission depletion (STED) and two-photon excitation (2PE) fluorescence microscopy with continuous wave (CW) laser beam using a new generation laser scanning confocal microscope equipped for STED-CW (TCS STED-CW, Leica Microsystems, Mannheim, Germany). We show the possibility to achieve CW-2PE with the very same beam used for STED-CW. This feature extends the performance of the microscope allowing multimodal imaging (CW-2PE, STED-CW, confocal).Mesh:
Year: 2011 PMID: 22171566 DOI: 10.1111/j.1365-2818.2011.03577.x
Source DB: PubMed Journal: J Microsc ISSN: 0022-2720 Impact factor: 1.758