Literature DB >> 22171566

Fast scanning STED and two-photon fluorescence excitation microscopy with continuous wave beam.

P Bianchini1, A Diaspro.   

Abstract

In this paper we report stimulated emission depletion (STED) and two-photon excitation (2PE) fluorescence microscopy with continuous wave (CW) laser beam using a new generation laser scanning confocal microscope equipped for STED-CW (TCS STED-CW, Leica Microsystems, Mannheim, Germany). We show the possibility to achieve CW-2PE with the very same beam used for STED-CW. This feature extends the performance of the microscope allowing multimodal imaging (CW-2PE, STED-CW, confocal).
© 2011 The Authors Journal of Microscopy © 2011 Royal Microscopical Society.

Mesh:

Year:  2011        PMID: 22171566     DOI: 10.1111/j.1365-2818.2011.03577.x

Source DB:  PubMed          Journal:  J Microsc        ISSN: 0022-2720            Impact factor:   1.758


  4 in total

1.  Tuning donut profile for spatial resolution in stimulated emission depletion microscopy.

Authors:  Bhanu Neupane; Fang Chen; Wei Sun; Daniel T Chiu; Gufeng Wang
Journal:  Rev Sci Instrum       Date:  2013-04       Impact factor: 1.523

Review 2.  Advanced fluorescence microscopy techniques--FRAP, FLIP, FLAP, FRET and FLIM.

Authors:  Hellen C Ishikawa-Ankerhold; Richard Ankerhold; Gregor P C Drummen
Journal:  Molecules       Date:  2012-04-02       Impact factor: 4.411

3.  Exploration of the two-photon excitation spectrum of fluorescent dyes at wavelengths below the range of the Ti:Sapphire laser.

Authors:  J Trägårdh; G Robb; R Amor; W B Amos; J Dempster; G McConnell
Journal:  J Microsc       Date:  2015-05-06       Impact factor: 1.758

4.  Achieving high-efficiency emission depletion nanoscopy by employing cross relaxation in upconversion nanoparticles.

Authors:  Qiuqiang Zhan; Haichun Liu; Baoju Wang; Qiusheng Wu; Rui Pu; Chao Zhou; Bingru Huang; Xingyun Peng; Hans Ågren; Sailing He
Journal:  Nat Commun       Date:  2017-10-20       Impact factor: 14.919

  4 in total

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