Literature DB >> 2217119

Characterization and purification of culture-derived soluble glycoproteins of Babesia canis.

G Azzar1, J Radisson, R Got.   

Abstract

The addition of [14C]-glucosamine to media of Babesia canis cultures causes the appearance of labeled glycoproteins in the culture supernatants. These radioactive soluble glycoproteins were separated according to their molecular weight by gel filtration and according to their (acidic) pI by preparative electrofocusing. The labeled fractions were then analyzed by SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis). The results showed three series of glycoproteic antigens. The molecular weights for the three antigens determined by gel filtration and by SDS-PAGE were approximately 100, 40, and 12.5 kDa, and the preparative gel electrofocusing suggested that the antigens focus in the pH range of 3-5.

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Year:  1990        PMID： 2217119     DOI： 10.1007/bf00932565

Source DB:  PubMed          Journal:  Parasitol Res        ISSN： 0932-0113            Impact factor:   2.289


  6 in total

1.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

2.  Bovine babesiosis: partial purification and characterization of blood culture-derived Babesia bovis.

Authors:  S M Gravely; R D Smith; E E Erp; G J Cantó; M Aikawa; B M Osorno; M Ristic
Journal:  Int J Parasitol       Date:  1979-12       Impact factor: 3.981

Review 3.  An update of the isolation and characterization of culture-derived soluble antigens of Babesia bovis.

Authors:  M A James
Journal:  Vet Parasitol       Date:  1984-06       Impact factor: 2.738

4.  Babesia bovis: continuous cultivation in a microaerophilous stationary phase culture.

Authors:  M G Levy; M Ristic
Journal:  Science       Date:  1980-03-14       Impact factor: 47.728

5.  Variable and common antigens of Babesia bovis parasites differing in strain and virulence.

Authors:  L P Kahl; R F Anders; B J Rodwell; P Timms; G F Mitchell
Journal:  J Immunol       Date:  1982-10       Impact factor: 5.422

6.  Bovine babesiosis: protection of cattle with culture-derived soluble Babesia bovis antigen.

Authors:  R D Smith; M A James; M Ristic; M Aikawa; C A Vega y Murguia
Journal:  Science       Date:  1981-04-17       Impact factor: 47.728
  6 in total
  2 in total

1.  Immune Response of Mice Against Babesia canis Antigens is Enhanced When Antigen is Coupled to Gold Nanoparticles.

Authors:  S A Staroverov; A S Fomin; S V Kozlov; A A Volkov; E S Kozlov; K P Gabalov; L A Dykman
Journal:  Acta Parasitol       Date:  2020-11-09       Impact factor: 1.440

2.  Characterization and comparison of merozoite antigens of different Babesia canis isolates by serological and immunological investigations.

Authors:  S Hauschild; P Shayan; E Schein
Journal:  Parasitol Res       Date:  1995       Impact factor: 2.289
  2 in total

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