AIMS: There is consistent lack of data focusing the topoisomerase-IIα gene status in lobular breast carcinoma, a subtype that usually shows poor responsiveness to chemotherapies including those using anthracycline drugs. METHODS AND RESULTS: Forty-six infiltrative lobular carcinomas, 13 with matched metastases, were used. Topoisomerase-IIα gene amplification was evaluated by chromogenic in situ hybridization (CISH) and fluorescence in situ hybridization (FISH). We also assessed Her2/neu status by CISH, FISH and silver in situ hybridization (SISH). HER2 immunoexpression was assessed by the HercepTest. Forty-four of 46 (95%) cases revealed no topoisomerase-IIα amplification, whereas two of 46 (5%) cases were amplified by all three techniques. Eleven of the 13 metastatic sites showed no amplification either in the primary or in the metastases (85%); the remaining two were amplified (15%). Her2/neu was not amplified in 44 of 46 (95%) cases nor was it amplified in 11 of 13 (95%) metastatic tissues. The two cases showing Her2/neu and topoisomerase-IIα amplification scored 3+; the remaining non-amplified cases scored 0 or 1+ in 40 and 2+ in four cases. CONCLUSIONS: In the era of personalized and tailored therapies, we suggest that patients affected by the classical lobular subtype of breast carcinoma constantly lack the ad hoc predictive rationale for receiving common chemotherapy that includes anthracyclines.
AIMS: There is consistent lack of data focusing the topoisomerase-IIα gene status in lobular breast carcinoma, a subtype that usually shows poor responsiveness to chemotherapies including those using anthracycline drugs. METHODS AND RESULTS: Forty-six infiltrative lobular carcinomas, 13 with matched metastases, were used. Topoisomerase-IIα gene amplification was evaluated by chromogenic in situ hybridization (CISH) and fluorescence in situ hybridization (FISH). We also assessed Her2/neu status by CISH, FISH and silver in situ hybridization (SISH). HER2 immunoexpression was assessed by the HercepTest. Forty-four of 46 (95%) cases revealed no topoisomerase-IIα amplification, whereas two of 46 (5%) cases were amplified by all three techniques. Eleven of the 13 metastatic sites showed no amplification either in the primary or in the metastases (85%); the remaining two were amplified (15%). Her2/neu was not amplified in 44 of 46 (95%) cases nor was it amplified in 11 of 13 (95%) metastatic tissues. The two cases showing Her2/neu and topoisomerase-IIα amplification scored 3+; the remaining non-amplified cases scored 0 or 1+ in 40 and 2+ in four cases. CONCLUSIONS: In the era of personalized and tailored therapies, we suggest that patients affected by the classical lobular subtype of breast carcinoma constantly lack the ad hoc predictive rationale for receiving common chemotherapy that includes anthracyclines.
Authors: Eleonora Brunello; Matteo Brunelli; Giuseppe Bogina; Anna Caliò; Erminia Manfrin; Alessia Nottegar; Marco Vergine; Annamaria Molino; Emilio Bria; Francesco Massari; Giampaolo Tortora; Sara Cingarlini; Serena Pedron; Marco Chilosi; Giuseppe Zamboni; Keith Miller; Guido Martignoni; Franco Bonetti Journal: J Exp Clin Cancer Res Date: 2012-12-27
Authors: E Bria; S Pilotto; M Simbolo; M Fassan; G de Manzoni; L Carbognin; I Sperduti; M Brunelli; I Cataldo; A Tomezzoli; A Mafficini; G Turri; N Karachaliou; R Rosell; G Tortora; A Scarpa Journal: Sci Rep Date: 2016-03-10 Impact factor: 4.379
Authors: Dorien Lobbezoo; Wilfred Truin; Adri Voogd; Rudi Roumen; Gerard Vreugdenhil; Marcus Wouter Dercksen; Franchette van den Berkmortel; Tineke Smilde; Agnes van de Wouw; Roel van Kampen; Johanna van Riel; Natascha Peters; Petronella Peer; Vivianne C G Tjan-Heijnen Journal: Oncotarget Date: 2016-05-17