L Beekman1, T Tohver, R Léguillette. 1. Department of Veterinary Clinical and Diagnostic Sciences, University of Calgary, Calgary, Alberta, Canada.
Abstract
OBJECTIVES: The pathophysiology of inflammatory airway disease (IAD) is unknown, but in some cases involves the accumulation of mast cells, neutrophils, or both in the bronchoalveolar lavage fluid (BALF). The objective of this study was to characterize cytokine gene expression in the BALF cells of horses with IAD, including a comparison of cytokine gene expression between IAD horses with increased BALF mast cells (IAD-Mast) or neutrophils (IAD-Neutro). METHODS: The mRNA expression of IL-4, IFN-γ, IL-17, IL-8, IL-1β, IL-5, IL-6, IL-10, IL-12p35, and eotaxin-2 was studied by quantitative polymerase chain reaction (QPCR) with efficiency correction in BALF samples of 17 horses with IAD (IAD-total), also subcategorized as 8 IAD-Mast and 9 IAD-Neutro, and 10 controls. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as a reference gene. Relative expression software tool (REST) analysis provided ratios of expression, statistical analysis, and confidence intervals for the results. RESULTS: Compared with the control group, IL-5, IL-1β, IL-6, IL-8, and IL-10 mRNA expression was upregulated 3.5-, 3.4-, 2.8-, 2.2-, and 1.9-fold, respectively, in the IAD-total group. The IAD-Neutro group showed increased expression of IL-17, IL-8, and IL-5 (4.7-, 2.5-, and 2.9-fold, respectively) and a decreased expression of IL-4 (3.4-fold) compared with the IAD-Mast group. CONCLUSION: Cytokines from the Th2 family plays a key role in IAD and a different pathophysiology may be involved in mast cell versus neutrophil BALF accumulation in IAD horses.
OBJECTIVES: The pathophysiology of inflammatory airway disease (IAD) is unknown, but in some cases involves the accumulation of mast cells, neutrophils, or both in the bronchoalveolar lavage fluid (BALF). The objective of this study was to characterize cytokine gene expression in the BALF cells of horses with IAD, including a comparison of cytokine gene expression between IADhorses with increased BALF mast cells (IAD-Mast) or neutrophils (IAD-Neutro). METHODS: The mRNA expression of IL-4, IFN-γ, IL-17, IL-8, IL-1β, IL-5, IL-6, IL-10, IL-12p35, and eotaxin-2 was studied by quantitative polymerase chain reaction (QPCR) with efficiency correction in BALF samples of 17 horses with IAD (IAD-total), also subcategorized as 8 IAD-Mast and 9 IAD-Neutro, and 10 controls. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as a reference gene. Relative expression software tool (REST) analysis provided ratios of expression, statistical analysis, and confidence intervals for the results. RESULTS: Compared with the control group, IL-5, IL-1β, IL-6, IL-8, and IL-10 mRNA expression was upregulated 3.5-, 3.4-, 2.8-, 2.2-, and 1.9-fold, respectively, in the IAD-total group. The IAD-Neutro group showed increased expression of IL-17, IL-8, and IL-5 (4.7-, 2.5-, and 2.9-fold, respectively) and a decreased expression of IL-4 (3.4-fold) compared with the IAD-Mast group. CONCLUSION: Cytokines from the Th2 family plays a key role in IAD and a different pathophysiology may be involved in mast cell versus neutrophil BALF accumulation in IADhorses.
Authors: Ashley Houtsma; Daniela Bedenice; Nicola Pusterla; Brenna Pugliese; Samantha Mapes; Andrew M Hoffman; Julia Paxson; Elizabeth Rozanski; Jean Mukherjee; Margaret Wigley; Melissa R Mazan Journal: Multidiscip Respir Med Date: 2015-11-03
Authors: E A Richard; M Depecker; M Defontis; C Leleu; G Fortier; P-H Pitel; A Couroucé-Malblanc Journal: J Vet Intern Med Date: 2014-09-30 Impact factor: 3.333
Authors: Stephanie Bond; Renaud Léguillette; Eric A Richard; Laurent Couetil; Jean-Pierre Lavoie; James G Martin; R Scott Pirie Journal: J Vet Intern Med Date: 2018-10-07 Impact factor: 3.333