Bin Xu1, Zheng Feng, Xing-Jian Xu, Wei Hu. 1. National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Key Laboratory of Parasite and Vector Biology, MOH, WHO Collaborating Center for Malaria, Schistosomiasis and Filariasis, Shanghai 200025, China.
Abstract
OBJECTIVE: To develop an aetiology detection method with higher sensitivity and accuracy for schistosomiasis japonica. METHODS: The fresh fecal samples were collected from residents of a schistosomiasis japonica endemic village in Laozi County of Hubei Province. Two samples per person were collected in two consecutive days. Each sample was examined three times with Kato-Katz technique, then the negative cases were re-examined by the stool hatching test after the egg concentration with nylon-tissue bags. The detective rates and the relation with egg per gram (EPG) of different methods were analyzed and compared. The epidemiology data were collected as well. RESULTS: A total of 562 residents were examined by Kato-Katz technique, and 67 of them were positive. The samples of 495 negative persons were re-examined by using the hatching test after the egg concentration with nylon-tissue bags. Six persons were positive. The total number of positive cases was 73. The rates of Kato-Katz technique with three slides of one sample and six slides of two samples were 65.8% and 91.8%, respectively. The detective rate was increased with the increase of examining slides. The geometrical averages of EPG from one slide to six slides were 61.12, 31.92, 23.71, 20.88, 19.35 and 8.83, respectively. The accuracy of EPG increased with the increase of numbers of slides. CONCLUSION: Kato-Katz technique with six slides of two samples is applicable in diagnosis of schistosomiasis japonica in order to achieve higher detective rate and more accurate EPG.
OBJECTIVE: To develop an aetiology detection method with higher sensitivity and accuracy for schistosomiasis japonica. METHODS: The fresh fecal samples were collected from residents of a schistosomiasis japonica endemic village in Laozi County of Hubei Province. Two samples per person were collected in two consecutive days. Each sample was examined three times with Kato-Katz technique, then the negative cases were re-examined by the stool hatching test after the egg concentration with nylon-tissue bags. The detective rates and the relation with egg per gram (EPG) of different methods were analyzed and compared. The epidemiology data were collected as well. RESULTS: A total of 562 residents were examined by Kato-Katz technique, and 67 of them were positive. The samples of 495 negative persons were re-examined by using the hatching test after the egg concentration with nylon-tissue bags. Six persons were positive. The total number of positive cases was 73. The rates of Kato-Katz technique with three slides of one sample and six slides of two samples were 65.8% and 91.8%, respectively. The detective rate was increased with the increase of examining slides. The geometrical averages of EPG from one slide to six slides were 61.12, 31.92, 23.71, 20.88, 19.35 and 8.83, respectively. The accuracy of EPG increased with the increase of numbers of slides. CONCLUSION: Kato-Katz technique with six slides of two samples is applicable in diagnosis of schistosomiasis japonica in order to achieve higher detective rate and more accurate EPG.
Authors: David U Olveda; Yuesheng Li; Remigio M Olveda; Alfred K Lam; Thao N P Chau; Donald A Harn; Gail M Williams; Darren J Gray; Allen G P Ross Journal: Trop Med Surg Date: 2013-08-20