Sangita Jindal1, Keri Greenseid, Dara Berger, Nanette Santoro, Lubna Pal. 1. Division of Reproductive Endocrinology & Infertility, Department of Obstetrics, Gynecology & Women's Health, Albert Einstein College of Medicine, Bronx, NY, USA. sangita.k.jindal@gmail.com
Abstract
PURPOSE: A symbiotic relationship between ovarian granulosa cells (GC) and the developing oocyte is critical. Genetic modulations in GC's can lead to reproductive insufficiency, highlighting the role of GC's in reproductive competence. Utilizing gene expression analyses in cumulus GC's, we attempt to enhance our understanding of mechanisms that may contribute to poor reproductive capacity in young women with diminished ovarian reserve (DOR). METHODS: We measured gremlin 1 (GREM1) gene expression in GC's from infertile women <38 years undergoing in vitro fertilization in the context of DOR. RESULTS: GREM1, a member of the differential screening-selected gene aberrative in neuroblastoma (DAN) family of genes known for its highly regulated expression pattern during folliculogenesis and a downstream effecter of oocyte-derived growth and differentiation factor 9, was down-regulated 3-fold (-3.08) in women with DOR versus control; down-regulation was confirmed by qRT-PCR (-4.02). CONCLUSION: This is the first demonstration linking differential expression of Gremlin with etiology of infertility in women.
PURPOSE: A symbiotic relationship between ovarian granulosa cells (GC) and the developing oocyte is critical. Genetic modulations in GC's can lead to reproductive insufficiency, highlighting the role of GC's in reproductive competence. Utilizing gene expression analyses in cumulus GC's, we attempt to enhance our understanding of mechanisms that may contribute to poor reproductive capacity in young women with diminished ovarian reserve (DOR). METHODS: We measured gremlin 1 (GREM1) gene expression in GC's from infertile women <38 years undergoing in vitro fertilization in the context of DOR. RESULTS:GREM1, a member of the differential screening-selected gene aberrative in neuroblastoma (DAN) family of genes known for its highly regulated expression pattern during folliculogenesis and a downstream effecter of oocyte-derived growth and differentiation factor 9, was down-regulated 3-fold (-3.08) in women with DOR versus control; down-regulation was confirmed by qRT-PCR (-4.02). CONCLUSION: This is the first demonstration linking differential expression of Gremlin with etiology of infertility in women.
Authors: T Adriaenssens; S Wathlet; I Segers; G Verheyen; A De Vos; J Van der Elst; W Coucke; P Devroey; J Smitz Journal: Hum Reprod Date: 2010-03-13 Impact factor: 6.918
Authors: R A Anderson; R Sciorio; H Kinnell; R A L Bayne; K J Thong; P A de Sousa; S Pickering Journal: Reproduction Date: 2009-07-14 Impact factor: 3.906