An efficient analytical method for analysis of spirotetramat and its metabolite spirotetramat-enol by HPLC.

Spirotetramat is a new compound which belongs to the chemical class of ketoenols. As per the available literature analysis of spirotetramat and its metabolites spirotetramat-enol is carried out by high pressure liquid chromatograph with mass spectrometry (HPLC-MS/MS). In this study we have standardized a method where analysis of both the compounds is carried out by HPLC. The extraction and cleanup of spirotetramat and its metabolites spirotetramat-enol was carried out by QuEChERS method. The cleaned up residues were estimated by HPLC equipped with a photo diode array detector at a wavelength of 250 nm. The mobile phase used was acetonitrile: water at a proportion of 40:60. The limit of quantification (LOQ) of the method was 0.05 mg kg(-1) for both spirotetramat and its metabolite spirotetramat-enol. The recoveries of both the compounds at the LOQ level were in the range of 72.72%-86.76% from mango and 74.82% to 86.92% from cabbage.