AIM: To investigate the neuroprotective effect of glycyrrhizin (Gly) against the ischemic injury of rat spinal cord and the possible role of the nuclear protein high-mobility group box 1 (HMGB1) in the process. METHODS: Male Sprague-Dawley rats were subjected to 45 min aortic occlusion to induce transient lumbar spinal cord ischemia. The motor functions of the animals were assessed according to the modified Tarlov scale. The animals were sacrificed 72 h after reperfusion and the lumbar spinal cord segment (L2-L4) was taken out for histopathological examination and Western blotting analysis. Serum inflammatory cytokine and HMGB1 levels were analyzed using ELISA. RESULTS: Gly (6 mg/kg) administered intravenously 30 min before inducing the transient lumbar spinal cord ischemia significantly improved the hind-limb motor function scores, and reduced the number of apoptotic neurons, which was accompanied by reduced levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) in the plasma and injured spinal cord. Moreover, the serum HMGB1 level correlated well with the serum TNF-α, IL-1β and IL-6 levels during the time period of reperfusion. CONCLUSION: The results suggest that Gly can attenuate the transient spinal cord ischemic injury in rats via reducing inflammatory cytokines and inhibiting the release of HMGB1.
AIM: To investigate the neuroprotective effect of glycyrrhizin (Gly) against the ischemic injury of rat spinal cord and the possible role of the nuclear protein high-mobility group box 1 (HMGB1) in the process. METHODS: Male Sprague-Dawley rats were subjected to 45 min aortic occlusion to induce transient lumbar spinal cord ischemia. The motor functions of the animals were assessed according to the modified Tarlov scale. The animals were sacrificed 72 h after reperfusion and the lumbar spinal cord segment (L2-L4) was taken out for histopathological examination and Western blotting analysis. Serum inflammatory cytokine and HMGB1 levels were analyzed using ELISA. RESULTS:Gly (6 mg/kg) administered intravenously 30 min before inducing the transient lumbar spinal cord ischemia significantly improved the hind-limb motor function scores, and reduced the number of apoptotic neurons, which was accompanied by reduced levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) in the plasma and injured spinal cord. Moreover, the serum HMGB1 level correlated well with the serum TNF-α, IL-1β and IL-6 levels during the time period of reperfusion. CONCLUSION: The results suggest that Gly can attenuate the transient spinal cord ischemic injury in rats via reducing inflammatory cytokines and inhibiting the release of HMGB1.
Authors: Mark F Conrad; Emel A Ergul; Virendra I Patel; Matthew R Cambria; Glenn M Lamuraglia; Mirela Simon; Richard P Cambria Journal: J Vasc Surg Date: 2011-02-11 Impact factor: 4.268
Authors: S J Klebanoff; M A Vadas; J M Harlan; L H Sparks; J R Gamble; J M Agosti; A M Waltersdorph Journal: J Immunol Date: 1986-06-01 Impact factor: 5.422
Authors: Liqun Yang; Peter C Blumbergs; Nigel R Jones; Jim Manavis; Ghafar T Sarvestani; Mounir N Ghabriel Journal: Spine (Phila Pa 1976) Date: 2004-05-01 Impact factor: 3.468
Authors: Serdar Akgun; Atike Tekeli; Ozlem Kurtkaya; Ali Civelek; Selim C Isbir; Koray Ak; Sinan Arsan; Aydin Sav Journal: Eur J Cardiothorac Surg Date: 2004-01 Impact factor: 4.191
Authors: Arnt E Fiane; Vibeke Videm; Per S Lingaas; Lars Heggelund; Erik W Nielsen; Odd R Geiran; Michael Fung; Tom E Mollnes Journal: Circulation Date: 2003-08-04 Impact factor: 29.690
Authors: Ahmed M Abu El-Asrar; Mohammad Mairaj Siddiquei; Mohd Imtiaz Nawaz; Karel Geboes; Ghulam Mohammad Journal: Mediators Inflamm Date: 2014-03-10 Impact factor: 4.711