OBJECTIVE: Animal studies have allowed important insights into the role of the nitric oxide synthase (NOS) enzymes in atherosclerosis and hypertension, as well as in stroke. In this study we tested the hypothesis that the NOS1 and NOS3 genes, respectively encoding neuronal NOS (nNOS) and endothelial NOS (eNOS), influence stroke susceptibility and outcome after a stroke event. METHODS: We conducted a case-control association study in 551 ischemic stroke patients and 530 controls to assess the role of NOS1 and NOS3 variants in stroke susceptibility. The same genes were tested for association with stroke outcome in a subset of 431 patients. RESULTS: Four NOS1 single nucleotide polymorphisms (SNPs) (rs2293050, rs2139733, rs7308402 and rs1483757) and four haplotypes were significantly associated with stroke susceptibility after adjusting for demographic, clinical and life-style risk factors, and correcting for multiple testing using the false discovery rate (FDR) method (SNPs: 0.004<(uncorrected)P<0.007 and 0.036<FDR q<0.048; haplotypes: 0.001<(uncorrected)P<0.010 and 0.018<FDR q<0.032). NOS1 variants were not associated with stroke outcome. We did not find any evidence for a role of the NOS3 gene in stroke susceptibility or outcome. CONCLUSION: Our results highlight NOS1 as a susceptibility factor for stroke, but do not corroborate previous NOS3 association findings with stroke risk. nNOS is known to play a major role in atherosclerosis development and in blood flow regulation, and it is plausible that its influence in stroke may be mediated through these two main clinical risk factors.
OBJECTIVE: Animal studies have allowed important insights into the role of the nitric oxide synthase (NOS) enzymes in atherosclerosis and hypertension, as well as in stroke. In this study we tested the hypothesis that the NOS1 and NOS3 genes, respectively encoding neuronal NOS (nNOS) and endothelial NOS (eNOS), influence stroke susceptibility and outcome after a stroke event. METHODS: We conducted a case-control association study in 551 ischemic strokepatients and 530 controls to assess the role of NOS1 and NOS3 variants in stroke susceptibility. The same genes were tested for association with stroke outcome in a subset of 431 patients. RESULTS: Four NOS1 single nucleotide polymorphisms (SNPs) (rs2293050, rs2139733, rs7308402 and rs1483757) and four haplotypes were significantly associated with stroke susceptibility after adjusting for demographic, clinical and life-style risk factors, and correcting for multiple testing using the false discovery rate (FDR) method (SNPs: 0.004<(uncorrected)P<0.007 and 0.036<FDR q<0.048; haplotypes: 0.001<(uncorrected)P<0.010 and 0.018<FDR q<0.032). NOS1 variants were not associated with stroke outcome. We did not find any evidence for a role of the NOS3 gene in stroke susceptibility or outcome. CONCLUSION: Our results highlight NOS1 as a susceptibility factor for stroke, but do not corroborate previous NOS3 association findings with stroke risk. nNOS is known to play a major role in atherosclerosis development and in blood flow regulation, and it is plausible that its influence in stroke may be mediated through these two main clinical risk factors.
Authors: Danielle M Backes; Afshan Siddiq; David G Cox; Federico C F Calboli; J Michael Gaziano; Jing Ma; Meir Stampfer; David J Hunter; Carlos A Camargo; Dominique S Michaud Journal: J Neurooncol Date: 2013-03-25 Impact factor: 4.130
Authors: Carlos Cruchaga; John S K Kauwe; Petra Nowotny; Kelly Bales; Eve H Pickering; Kevin Mayo; Sarah Bertelsen; Anthony Hinrichs; Anne M Fagan; David M Holtzman; John C Morris; Alison M Goate Journal: Hum Mol Genet Date: 2012-07-20 Impact factor: 6.150
Authors: Regina F Nasyrova; Polina V Moskaleva; Elena E Vaiman; Natalya A Shnayder; Nataliya L Blatt; Albert A Rizvanov Journal: Int J Mol Sci Date: 2020-02-26 Impact factor: 5.923
Authors: Seung Don Yoo; Jun Sang Park; Dong Hwan Yun; Hee-Sang Kim; Su Kang Kim; Dong Hwan Kim; Jinmann Chon; Goun Je; Yoon-Seong Kim; Joo-Ho Chung; Seung Joon Chung; Jin Ah Yeo Journal: Ann Rehabil Med Date: 2016-02-26