INTRODUCTION: Endogenous dentin matrix metalloproteinases (MMPs) contribute to extracellular collagen matrix degradation in hybrid layers after adhesive dentin bonding procedures. Endodontic irrigants, including chlorhexidine and ethylenediaminetetraacetic acid (EDTA), might help protect the hybrid layer from this process. The objective of the present study was to determine the exposure time necessary for EDTA to inactivate endogenous MMP activity in human dentin. METHODS: Dentin beams (2 × 1 × 3 mm) were prepared from mid-coronal dentin of extracted third molars. The beams were demineralized in 10 wt% phosphoric acid, which also activated endogenous MMPs, and were divided into 4 experimental groups on the basis of exposure time to 17% EDTA (0, 1, 2, or 5 minutes). A generic colorimetric MMP assay measured MMP activity via absorbance at 412 nm. Data were evaluated by Kruskal-Wallis analysis of variance, followed by Dunn pair-wise comparisons at α = 0.05. RESULTS: All exposure times resulted in significant inhibition (P < .001) compared with unexposed controls. Specifically, percent inhibition for 1-, 2-, and 5-minute exposure times was 55.1% ± 21.5%, 72.8% ± 11.7%, and 74.7% ± 19.7%, respectively. CONCLUSIONS: Seventeen percent EDTA significantly inhibits endogenous MMP activity of human dentin within 1-2 minutes. This might minimize hybrid layer degradation after resin bonding procedures in the root canal space.
INTRODUCTION: Endogenous dentin matrix metalloproteinases (MMPs) contribute to extracellular collagen matrix degradation in hybrid layers after adhesive dentin bonding procedures. Endodontic irrigants, including chlorhexidine and ethylenediaminetetraacetic acid (EDTA), might help protect the hybrid layer from this process. The objective of the present study was to determine the exposure time necessary for EDTA to inactivate endogenous MMP activity in human dentin. METHODS: Dentin beams (2 × 1 × 3 mm) were prepared from mid-coronal dentin of extracted third molars. The beams were demineralized in 10 wt% phosphoric acid, which also activated endogenous MMPs, and were divided into 4 experimental groups on the basis of exposure time to 17% EDTA (0, 1, 2, or 5 minutes). A generic colorimetric MMP assay measured MMP activity via absorbance at 412 nm. Data were evaluated by Kruskal-Wallis analysis of variance, followed by Dunn pair-wise comparisons at α = 0.05. RESULTS: All exposure times resulted in significant inhibition (P < .001) compared with unexposed controls. Specifically, percent inhibition for 1-, 2-, and 5-minute exposure times was 55.1% ± 21.5%, 72.8% ± 11.7%, and 74.7% ± 19.7%, respectively. CONCLUSIONS: Seventeen percent EDTA significantly inhibits endogenous MMP activity of human dentin within 1-2 minutes. This might minimize hybrid layer degradation after resin bonding procedures in the root canal space.
Authors: Yoshihiro Nishitani; Masahiro Yoshiyama; Bakul Wadgaonkar; Lorenzo Breschi; Ferdinando Mannello; Annalisa Mazzoni; Ricardo M Carvalho; Leo Tjäderhane; Franklin R Tay; David H Pashley Journal: Eur J Oral Sci Date: 2006-04 Impact factor: 2.612
Authors: Jongryul Kim; Toshikazu Uchiyama; Marcela Carrilho; Kelli A Agee; Annalisa Mazzoni; Lorenzo Breschi; Ricardo M Carvalho; Leo Tjäderhane; Stephen Looney; Courtney Wimmer; Arzu Tezvergil-Mutluay; Franklin R Tay; David H Pashley Journal: Dent Mater Date: 2010-05-15 Impact factor: 5.304
Authors: Marcela R Carrilho; Ricardo M Carvalho; Ethan N Sousa; José Nicolau; Lorenzo Breschi; Annalisa Mazzoni; Leo Tjäderhane; Franklin R Tay; Kelli Agee; David H Pashley Journal: Dent Mater Date: 2010-05-15 Impact factor: 5.304
Authors: Arzu Tezvergil-Mutluay; Kelli A Agee; Tomohiro Hoshika; Marcela Carrilho; Lorenzo Breschi; Leo Tjäderhane; Yoshihiro Nishitani; Ricardo M Carvalho; Stephen Looney; Franklin R Tay; David H Pashley Journal: Dent Mater Date: 2010-08-04 Impact factor: 5.304
Authors: David H Pashley; Franklin R Tay; Lorenzo Breschi; Leo Tjäderhane; Ricardo M Carvalho; Marcela Carrilho; Arzu Tezvergil-Mutluay Journal: Dent Mater Date: 2010-11-27 Impact factor: 5.304
Authors: A Tezvergil-Mutluay; K A Agee; T Uchiyama; S Imazato; M M Mutluay; M Cadenaro; L Breschi; Y Nishitani; F R Tay; D H Pashley Journal: J Dent Res Date: 2011-01-06 Impact factor: 6.116
Authors: P Garnero; M Ferreras; M A Karsdal; R Nicamhlaoibh; J Risteli; O Borel; P Qvist; P D Delmas; N T Foged; J M Delaissé Journal: J Bone Miner Res Date: 2003-05 Impact factor: 6.741
Authors: Leo Tjäderhane; Fabio D Nascimento; Lorenzo Breschi; Annalisa Mazzoni; Ivarne L S Tersariol; Saulo Geraldeli; Arzu Tezvergil-Mutluay; Marcela Carrilho; Ricardo M Carvalho; Franklin R Tay; David H Pashley Journal: Dent Mater Date: 2013-08-14 Impact factor: 5.304
Authors: Christoph P Fik; Stefan Konieczny; David H Pashley; Christian J Waschinski; Reinhild S Ladisch; Ulrich Salz; Thorsten Bock; Joerg C Tiller Journal: Macromol Biosci Date: 2014-08-08 Impact factor: 4.979
Authors: Martha Goël Brackett; Kelli A Agee; William W Brackett; William O Key; Camila Sabatini; Melissa T Kato; Marilia A R Buzalaf; Leo Tjäderhane; David H Pashley Journal: J Nat Sci Date: 2015-06
Authors: Ana Coelho; Inês Amaro; Ana Apolónio; Anabela Paula; José Saraiva; Manuel Marques Ferreira; Carlos Miguel Marto; Eunice Carrilho Journal: Int J Mol Sci Date: 2021-04-22 Impact factor: 5.923