W Han1, Z Wang, X Lu, C Guo. 1. Department of Gastroenterology, Qilu Hospital of Shandong University, Jinan, Shandong Province, China.
Abstract
BACKGROUND: Growing evidence suggests that protease activated receptors (PARs) are mediators of persistent neuropathic pain, but their possible function as mediators in patients with post infectious irritable bowel syndrome (PI-IBS) remains to be further explored. This article aims to investigate the expression of PAR(2) and PAR(4) in the colonic mucosa of patients with PI-IBS, focusing on correlation with mast cell activation status. METHODS: A total of 17 normal controls and 23 patients with PI-IBS volunteered the study. The expression and localization of PAR(2) and PAR(4) were investigated by RT-PCR and immunohistochemistry, and the expression of PAR(2) and PAR(4) in the mast cells was examined using double-immunofluorescence staining. KEY RESULTS: The immunohistochemical study revealed that epithelial and submucosal cells showed immunoreactivity for both PAR(2) and PAR(4). Protease activated receptor 4 mRNA expression and immunoreactivity were down-regulated in PI-IBS compared with the control group. Specifically, a reduced immunoreactivity for PAR(4) was observed in mast cells of PI-IBS compared with normal controls, whereas there are no significant differences shown in PAR(2) between the PI-IBS and the control group. It is also found that the PAR(4) immunoreactivity decreases, while the activity of mast cells increases in PI-IBS rather than normal controls. CONCLUSIONS & INFERENCES: This study outlines the down-regulation of PAR(4) in the mast cells of PI-IBS. It could be of considerable interests in understanding the mechanisms involved in the persistent colonic hypersensitivity and their potential role as therapeutic targets for PI-IBS.
BACKGROUND: Growing evidence suggests that protease activated receptors (PARs) are mediators of persistent neuropathic pain, but their possible function as mediators in patients with post infectious irritable bowel syndrome (PI-IBS) remains to be further explored. This article aims to investigate the expression of PAR(2) and PAR(4) in the colonic mucosa of patients with PI-IBS, focusing on correlation with mast cell activation status. METHODS: A total of 17 normal controls and 23 patients with PI-IBS volunteered the study. The expression and localization of PAR(2) and PAR(4) were investigated by RT-PCR and immunohistochemistry, and the expression of PAR(2) and PAR(4) in the mast cells was examined using double-immunofluorescence staining. KEY RESULTS: The immunohistochemical study revealed that epithelial and submucosal cells showed immunoreactivity for both PAR(2) and PAR(4). Protease activated receptor 4 mRNA expression and immunoreactivity were down-regulated in PI-IBS compared with the control group. Specifically, a reduced immunoreactivity for PAR(4) was observed in mast cells of PI-IBS compared with normal controls, whereas there are no significant differences shown in PAR(2) between the PI-IBS and the control group. It is also found that the PAR(4) immunoreactivity decreases, while the activity of mast cells increases in PI-IBS rather than normal controls. CONCLUSIONS & INFERENCES: This study outlines the down-regulation of PAR(4) in the mast cells of PI-IBS. It could be of considerable interests in understanding the mechanisms involved in the persistent colonic hypersensitivity and their potential role as therapeutic targets for PI-IBS.
Authors: Min A Jhun; Jennifer A Smith; Erin B Ware; Sharon L R Kardia; Thomas H Mosley; Stephen T Turner; Patricia A Peyser; Sung Kyun Park Journal: Am J Epidemiol Date: 2017-11-15 Impact factor: 4.897
Authors: Hannah Ceuleers; Nikita Hanning; Jelena Heirbaut; Samuel Van Remoortel; Jurgen Joossens; Pieter Van Der Veken; Sven M Francque; Michelle De Bruyn; Anne-Marie Lambeir; Joris G De Man; Jean-Pierre Timmermans; Koen Augustyns; Ingrid De Meester; Benedicte Y De Winter Journal: Br J Pharmacol Date: 2018-07-23 Impact factor: 8.739
Authors: Eliziane S Patricio; Robson Costa; Claudia P Figueiredo; Katharina Gers-Barlag; Maíra A Bicca; Marianne N Manjavachi; Gabriela C Segat; Clive Gentry; Ana P Luiz; Elizabeth S Fernandes; Thiago M Cunha; Stuart Bevan; João B Calixto Journal: J Invest Dermatol Date: 2015-05-08 Impact factor: 8.551
Authors: Hannah Ceuleers; Hanne Van Spaendonk; Nikita Hanning; Jelena Heirbaut; Anne-Marie Lambeir; Jurgen Joossens; Koen Augustyns; Joris G De Man; Ingrid De Meester; Benedicte Y De Winter Journal: World J Gastroenterol Date: 2016-12-21 Impact factor: 5.742