AIM: To investigate the role of β-catenin in pathogenesis of nasopharyngeal carcinoma (NPC). METHODS: Cellular proliferation, apoptosis, matrix penetration assay, and western blotting were employed to determine cell biological changes in NPC cell lines transfected with β-catenin siRNA. Immunohistochemistry staining was used to detect β-catenin and Ki-67 expression in NPC tissue. RESULTS: β-Catenin was upregulated in NPC cell lines and tissues compared with chronic nasopharyngitis tissue. β-Catenin knockdown dramatically inhibited cellular growth, migration and invasion, but induced apoptosis of NPC cells. Further study showed that downstream genes of β-catenin signaling pathway including cyclin D1, c-Myc, MMP2 and MMP9 expression were suppressed in NPC cell lines transfected with β-catenin siRNA. CONCLUSION: Targeting β-catenin signaling pathway may be a noval strategy for NPC therapy. Copyright Â
AIM: To investigate the role of β-catenin in pathogenesis of nasopharyngeal carcinoma (NPC). METHODS: Cellular proliferation, apoptosis, matrix penetration assay, and western blotting were employed to determine cell biological changes in NPC cell lines transfected with β-catenin siRNA. Immunohistochemistry staining was used to detect β-catenin and Ki-67 expression in NPC tissue. RESULTS: β-Catenin was upregulated in NPC cell lines and tissues compared with chronic nasopharyngitis tissue. β-Catenin knockdown dramatically inhibited cellular growth, migration and invasion, but induced apoptosis of NPC cells. Further study showed that downstream genes of β-catenin signaling pathway including cyclin D1, c-Myc, MMP2 and MMP9 expression were suppressed in NPC cell lines transfected with β-catenin siRNA. CONCLUSION: Targeting β-catenin signaling pathway may be a noval strategy for NPC therapy. Copyright Â
Authors: Mijung Kwon; Soo Jin Lee; Yarong Wang; Yevangelina Rybak; Alex Luna; Srilakshmi Reddy; Asha Adem; Brian T Beaty; John S Condeelis; Steven K Libutti Journal: Int J Cancer Date: 2014-02-20 Impact factor: 7.396