Molecular characterization of two recombinant potato virus Y isolates from China.

Isolates of potato virus Y (PVY) have been divided into several strains. We determined the genomic sequences of PVY isolates AQ4 and FZ10 from tobacco in China. AQ4 and FZ10 had genome of 9700 and 9698 nucleotides, respectively. In phylogenetic analysis of complete genome sequences, AQ4 was clustered with strain N-Wi, and FZ10 with NTN. AQ4 had two recombination sites within the P1 and P3 genes, while FZ10 had three within the P1, P3 and NIa-Pro genes. When compared to typical NTN isolates, FZ10 lacked a recombination site within the CP gene and, thus, represents a novel recombination type of PVY.