| Literature DB >> 22138638 |
Kristen L Koterba1, Trissa Borgschulte, Michael W Laird.
Abstract
During large-scale manufacturing of an IgG1 monoclonal antibody in Chinese hamster ovary (CHO) cells, reduction of the antibody's disulfide bonds was observed. We present evidence that mammalian thioredoxin 1 (TXN1) is the terminal enzyme responsible for this reduction event. We demonstrate a marked prevention of IgG1 disulfide bond reduction in a cell-density dependent manner by knocking down expression of TXN1 via lentivirus transduction of short hairpin RNA.Entities:
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Year: 2011 PMID： 22138638 DOI： 10.1016/j.jbiotec.2011.11.009
Source DB: PubMed Journal: J Biotechnol ISSN： 0168-1656 Impact factor: 3.307