OBJECTIVES: This study describes the distribution of dystroglycan (DG) in human placenta, membranes, and uterine decidua. STUDY DESIGN: Dystroglycan expression was characterized by Western blotting, immunohistochemistry, and immunofluorescence microscopy using human tissues and cultured cells. RESULTS: Both α-DG and β-DG are expressed in the term syncytiotrophoblast, and α-DG is localized to the basement membrane. In first-trimester chorionic villi, α-DG and β-DG are localized to the periphery of the cytotrophoblast. Expression varies in term fetal membranes. α-Dystroglycan is not detectable in choriocarcinoma cells or HTR cells, but β-DG is present in both normal and cleaved forms. CONCLUSION: Dystroglycan is expressed at high levels in human trophoblasts, and localization of the α- and β-subunits varies with gestational age and trophoblast differentiation. Because DG expression inversely correlates with invasiveness in many cancers, its pattern of expression in trophoblasts suggests a possible function in inhibition of placental invasion.
OBJECTIVES: This study describes the distribution of dystroglycan (DG) in human placenta, membranes, and uterine decidua. STUDY DESIGN:Dystroglycan expression was characterized by Western blotting, immunohistochemistry, and immunofluorescence microscopy using human tissues and cultured cells. RESULTS: Both α-DG and β-DG are expressed in the term syncytiotrophoblast, and α-DG is localized to the basement membrane. In first-trimester chorionic villi, α-DG and β-DG are localized to the periphery of the cytotrophoblast. Expression varies in term fetal membranes. α-Dystroglycan is not detectable in choriocarcinoma cells or HTR cells, but β-DG is present in both normal and cleaved forms. CONCLUSION:Dystroglycan is expressed at high levels in human trophoblasts, and localization of the α- and β-subunits varies with gestational age and trophoblast differentiation. Because DG expression inversely correlates with invasiveness in many cancers, its pattern of expression in trophoblasts suggests a possible function in inhibition of placental invasion.
Authors: R A Williamson; M D Henry; K J Daniels; R F Hrstka; J C Lee; Y Sunada; O Ibraghimov-Beskrovnaya; K P Campbell Journal: Hum Mol Genet Date: 1997-06 Impact factor: 6.150
Authors: C Losasso; F Di Tommaso; A Sgambato; R Ardito; A Cittadini; B Giardina; T C Petrucci; A Brancaccio Journal: FEBS Lett Date: 2000-11-10 Impact factor: 4.124
Authors: Daniel E Michele; Rita Barresi; Motoi Kanagawa; Fumiaki Saito; Ronald D Cohn; Jakob S Satz; James Dollar; Ichizo Nishino; Richard I Kelley; Hannu Somer; Volker Straub; Katherine D Mathews; Steven A Moore; Kevin P Campbell Journal: Nature Date: 2002-07-25 Impact factor: 49.962
Authors: Jarnail Singh; Yoko Itahana; Selena Knight-Krajewski; Motoi Kanagawa; Kevin P Campbell; Mina J Bissell; John Muschler Journal: Cancer Res Date: 2004-09-01 Impact factor: 12.701