ETHNOPHARMACOLOGICAL RELEVANCE: The leaves of Michauxia species are used for the treatment of wounds in Turkish traditional medicine. In the present study, wound healing, anti-inflammatory and antioxidant activities of the extracts obtained from the root and herb of 5 species of Michauxia collected in different parts of Turkey were evaluated. MATERIAL AND METHODS: In vivo incision and excision wound models were used in order to assess the wound healing effects of the methanolic extracts of the plants. Skin samples were also evaluated histopathologically. In vivo inhibitory effect of the extracts on acetic acid-induced increase in capillary permeability was studied for the assessment of anti-inflammatory activity. TBA (thiobarbituric acid) test, qualitative and quantitative DPPH (2,2-diphenyl-1-picrylhydrazyl) tests were used to evaluate the antioxidant activity. RESULTS: Noteworthy wound healing activity was observed for the ointment formulation prepared with 1% Michauxia nuda (root) and Michauxia tchihatchewii (herb) extracts. The results of histopathological evaluation supported the outcome of incision and excision wound models. Moreover, the Michauxia nuda (root) exerted remarkable anti-inflammatory effect. The highest antioxidant activity was observed with the ethyl acetate extract of Michauxia tchihatchewii herb. CONCLUSION: The experimental study revealed that Michauxia displays remarkable wound healing and anti-inflammatory and antioxidant activities.
ETHNOPHARMACOLOGICAL RELEVANCE: The leaves of Michauxia species are used for the treatment of wounds in Turkish traditional medicine. In the present study, wound healing, anti-inflammatory and antioxidant activities of the extracts obtained from the root and herb of 5 species of Michauxia collected in different parts of Turkey were evaluated. MATERIAL AND METHODS: In vivo incision and excision wound models were used in order to assess the wound healing effects of the methanolic extracts of the plants. Skin samples were also evaluated histopathologically. In vivo inhibitory effect of the extracts on acetic acid-induced increase in capillary permeability was studied for the assessment of anti-inflammatory activity. TBA (thiobarbituric acid) test, qualitative and quantitative DPPH (2,2-diphenyl-1-picrylhydrazyl) tests were used to evaluate the antioxidant activity. RESULTS: Noteworthy wound healing activity was observed for the ointment formulation prepared with 1% Michauxia nuda (root) and Michauxia tchihatchewii (herb) extracts. The results of histopathological evaluation supported the outcome of incision and excision wound models. Moreover, the Michauxia nuda (root) exerted remarkable anti-inflammatory effect. The highest antioxidant activity was observed with the ethyl acetate extract of Michauxia tchihatchewii herb. CONCLUSION: The experimental study revealed that Michauxia displays remarkable wound healing and anti-inflammatory and antioxidant activities.
Authors: Lucas Fernando Sérgio Gushiken; Carlos Alberto Hussni; Jairo Kenupp Bastos; Ariane Leite Rozza; Fernando Pereira Beserra; Ana Júlia Vieira; Carlos Roberto Padovani; Marivane Lemos; Maurilio Polizello Junior; Jonas Joaquim Mangabeira da Silva; Rafael Henrique Nóbrega; Emanuel Ricardo Monteiro Martinez; Cláudia Helena Pellizzon Journal: Evid Based Complement Alternat Med Date: 2017-08-27 Impact factor: 2.629