Literature DB >> 22135638

Isolation and molecular characterisation of Mycobacterium bovis from raw milk in Tunisia.

I Ben Kahla1, M L Boschiroli, F Souissi, N Cherif, M Benzarti, J Boukadida, S Hammami.   

Abstract

BACKGROUND: Consumption of raw milk and unpasteurized dairy products is common in Tunisia where bovine tuberculosis remains enzootic. We herein investigated the frequency of M. bovis isolation from raw milk.
METHODS: Three hundred and six milk samples collected from 102 infected cows in different Tunisian regions were analysed. M. bovis isolates were further characterized by spoligotyping and variable number tandem repeat typing.
RESULTS: A total of five (4.9 %) M. bovis strains exhibiting three different genotypes were isolated.
CONCLUSION: This study demonstrates that consumers of raw milk or derivatives in Tunisia are at high risk of zoonotic infection with M. bovis.

Entities:  

Keywords:  Mycobacterium bovis; Raw milk; Tuberculosis; Zoonosis

Mesh:

Year:  2011        PMID: 22135638      PMCID: PMC3220130          DOI: 10.4314/ahs.v11i3.70032

Source DB:  PubMed          Journal:  Afr Health Sci        ISSN: 1680-6905            Impact factor:   0.927


  10 in total

1.  PCR-based method to differentiate the subspecies of the Mycobacterium tuberculosis complex on the basis of genomic deletions.

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Journal:  J Clin Microbiol       Date:  2003-04       Impact factor: 5.948

2.  Direct detection and identification of Mycobacterium tuberculosis and Mycobacterium bovis in bovine samples by a novel nested PCR assay: correlation with conventional techniques.

Authors:  A Mishra; A Singhal; D S Chauhan; V M Katoch; K Srivastava; S S Thakral; S S Bharadwaj; V Sreenivas; H K Prasad
Journal:  J Clin Microbiol       Date:  2005-11       Impact factor: 5.948

3.  Development of variable-number tandem repeat typing of Mycobacterium bovis: comparison of results with those obtained by using existing exact tandem repeats and spoligotyping.

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4.  Spacer oligonucleotide typing of Mycobacterium bovis strains from cattle and other animals: a tool for studying epidemiology of tuberculosis.

Authors:  A Aranaz; E Liébana; A Mateos; L Dominguez; D Vidal; M Domingo; O Gonzolez; E F Rodriguez-Ferri; A E Bunschoten; J D Van Embden; D Cousins
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5.  Detection of Mycobacterium tuberculosis complex in cattle by PCR using milk, lymph node aspirates, and nasal swabs.

Authors:  F Vitale; G Capra; L Maxia; S Reale; G Vesco; S Caracappa
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Review 6.  The importance of Mycobacterium bovis as a zoonosis.

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Journal:  Vet Microbiol       Date:  2006-01-18       Impact factor: 3.293

7.  Isolation of Mycobacterium bovis & M. tuberculosis from cattle of some farms in north India--possible relevance in human health.

Authors:  K Srivastava; D S Chauhan; P Gupta; H B Singh; V D Sharma; V S Yadav; S S Thakral; J S Dharamdheeran; P Nigam; H K Prasad; V M Katoch
Journal:  Indian J Med Res       Date:  2008-07       Impact factor: 2.375

Review 8.  Zoonotic tuberculosis due to Mycobacterium bovis in developing countries.

Authors:  O Cosivi; J M Grange; C J Daborn; M C Raviglione; T Fujikura; D Cousins; R A Robinson; H F Huchzermeyer; I de Kantor; F X Meslin
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  10 in total
  16 in total

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4.  Longevity of Mycobacterium bovis in Raw and Traditional Souring Milk as a Function of Storage Temperature and Dose.

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Review 5.  Diagnosis of mycobacteria in bovine milk: an overview.

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8.  Genetic diversity assessment of Tunisian Mycobacterium bovis population isolated from cattle.

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9.  Genetic diversity and potential routes of transmission of Mycobacterium bovis in Mozambique.

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10.  Nontuberculous mycobacteria in milk from positive cows in the intradermal comparative cervical tuberculin test: implications for human tuberculosis infections.

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