Literature DB >> 22130934

Extraction of nucleic acids from bone.

Alun Hughes1, Tracy L Stewart, Val Mann.   

Abstract

Here, we present methods for extracting DNA and RNA from samples of whole bone tissue and culture bone cells and describe methods quantitative and qualitative measurement of the extracted nucleic acids. These protocols described provide high-quality nucleic acids suitable for downstream applications such as quantitative PCR and microarrays.

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Year:  2012        PMID： 22130934     DOI： 10.1007/978-1-61779-415-5_17

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  4 in total

1.  A novel methodological approach to simultaneously extract high-quality total RNA and proteins from cortical and trabecular bone.

Authors:  Martina Faraldi; Laura Mangiavini; Caterina Conte; Giuseppe Banfi; Nicola Napoli; Giovanni Lombardi
Journal:  Open Biol       Date:  2022-05-04       Impact factor: 7.124

2.  An Improved Method for Isolating High-Quality RNA From Rat Bone at Room Temperature Without the Need for Specialized Equipment.

Authors:  Frideriki Poutoglidou; Athanasios Saitis; Chryssa Pourzitaki; Dimitrios Kouvelas
Journal:  Cureus       Date:  2021-03-10

3.  Comparison of Different Fixation Methods for Combined Histological and Biomolecular Analysis of Fixed and Decalcified Bone Samples.

Authors:  Sarah Al-Maawi; Priscilia Valenzuela; Eva Dohle; Anja Heselich; Robert Sader; Shahram Ghanaati
Journal:  Methods Protoc       Date:  2022-07-21

4.  Atherosclerosis and Bone Loss in Humans-Results From Deceased Donors and From Patients Submitted to Carotid Endarterectomy.

Authors:  Diana Carmona-Fernandes; Sofia C Barreira; Natacha Leonardo; Renata I Casimiro; Alice M Castro; Pedro Oliveira Santos; António N Fernandes; Filipe Cortes-Figueiredo; Carolina A Gonçalves; Rafael Cruz; Mariana L Fernandes; Margarida Ivo; Luis M Pedro; Helena Canhão; João Eurico Fonseca; Maria José Santos
Journal:  Front Med (Lausanne)       Date:  2021-05-20
  4 in total

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