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Literature DB >> 22130928

Generation of human osteoclasts from peripheral blood.

Kim Henriksen¹, Morten A Karsdal, Adam Taylor, Denise Tosh, Fraser P Coxon.

Abstract

Osteoclasts are multi-nucleated cells that have the unique ability to resorb calcified bone matrix. They derive from haematopoietic precursor cells, and can be generated in vitro by stimulation of peripheral blood mononuclear cells with the cytokines M-CSF and RANKL. In this chapter, we describe the method for generating human osteoclast from peripheral blood or buffy coats, as well as methods for studying both the differentiation and resorbing activity of these cells.

Entities: Chemical

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Year: 2012 PMID： 22130928 DOI： 10.1007/978-1-61779-415-5_11

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

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Cited

10 in total

1. IL-27 Modulates the Cytokine Secretion in the T Cell-Osteoclast Crosstalk During HIV Infection.

Authors: Tong Li; Colleen Hadigan; Jarred M Whitlock; Jing Qin; Jai Kumar; Princy Kumar; Marta Catalfamo
Journal: Front Immunol Date: 2022-04-05 Impact factor: 8.786

2. Aging and menopause reprogram osteoclast precursors for aggressive bone resorption.

Authors: Anaïs Marie Julie Møller; Jean-Marie Delaissé; Jacob Bastholm Olesen; Jonna Skov Madsen; Luisa Matos Canto; Troels Bechmann; Silvia Regina Rogatto; Kent Søe
Journal: Bone Res Date: 2020-07-01 Impact factor: 13.567

3. Generation and culture of osteoclasts.

Authors: Silvia Marino; John G Logan; David Mellis; Mattia Capulli
Journal: Bonekey Rep Date: 2014-09-10

4. An Optimized Method to Generate Human Active Osteoclasts From Peripheral Blood Monocytes.

Authors: Dina Abdallah; Marie-Laure Jourdain; Julien Braux; Christine Guillaume; Sophie C Gangloff; Jacky Jacquot; Frédéric Velard
Journal: Front Immunol Date: 2018-04-04 Impact factor: 7.561

5. Aging and menopause reprogram osteoclast precursors for aggressive bone resorption.

6. Establishment and validation of an in vitro co-culture model for oral cell lines using human PBMC-derived osteoclasts, osteoblasts, fibroblasts and keratinocytes.

Authors: Daniel Steller; Alexandra Scheibert; Tabea Sturmheit; Samer G Hakim
Journal: Sci Rep Date: 2020-10-08 Impact factor: 4.379

Generation of human osteoclasts from peripheral blood.

1. IL-27 Modulates the Cytokine Secretion in the T Cell-Osteoclast Crosstalk During HIV Infection.

2. Aging and menopause reprogram osteoclast precursors for aggressive bone resorption.

3. Generation and culture of osteoclasts.

4. An Optimized Method to Generate Human Active Osteoclasts From Peripheral Blood Monocytes.

5. Aging and menopause reprogram osteoclast precursors for aggressive bone resorption.

6. Establishment and validation of an in vitro co-culture model for oral cell lines using human PBMC-derived osteoclasts, osteoblasts, fibroblasts and keratinocytes.

Review 7. Cell Sources for Human In vitro Bone Models.

8. Activated γδ T cells inhibit osteoclast differentiation and resorptive activity in vitro.

9. Osteoclasts degrade bone and cartilage knee joint compartments through different resorption processes.

Review 10. In vitro Models of Bone Remodelling and Associated Disorders.