Literature DB >> 22130923

Studying osteocyte function using the cell lines MLO-Y4 and MLO-A5.

Jennifer Rosser1, Lynda F Bonewald.   

Abstract

We describe the culture and use of MLO-Y4 cells in studies of gene expression, response to fluid flow, and dendrite growth. We also describe how to use the MLO-A5 cells as a model of osteoblast to osteocyte -differentiation and how to study their mineralization. These studies serve as a beginning point to study osteocyte functions and molecular mechanisms responsible for these functions.

Mesh:

Substances:

Year:  2012        PMID: 22130923     DOI: 10.1007/978-1-61779-415-5_6

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN: 1064-3745


  25 in total

Review 1.  Investigating Osteocytic Perilacunar/Canalicular Remodeling.

Authors:  Cristal S Yee; Charles A Schurman; Carter R White; Tamara Alliston
Journal:  Curr Osteoporos Rep       Date:  2019-08       Impact factor: 5.096

2.  Impact of Gap Junctional Intercellular Communication on MLO-Y4 Sclerostin and Soluble Factor Expression.

Authors:  S L York; P Sethu; M M Saunders
Journal:  Ann Biomed Eng       Date:  2015-07-08       Impact factor: 3.934

3.  A New Osteocytic Cell Line, Raising New Questions and Opportunities.

Authors:  Ivo Kalajzic
Journal:  J Bone Miner Res       Date:  2019-06-07       Impact factor: 6.741

Review 4.  The osteocyte: an endocrine cell ... and more.

Authors:  Sarah L Dallas; Matthew Prideaux; Lynda F Bonewald
Journal:  Endocr Rev       Date:  2013-04-23       Impact factor: 19.871

5.  Ex vivo construction of human primary 3D-networked osteocytes.

Authors:  Qiaoling Sun; Saba Choudhary; Ciaran Mannion; Yair Kissin; Jenny Zilberberg; Woo Y Lee
Journal:  Bone       Date:  2017-09-21       Impact factor: 4.398

Review 6.  Osteocytogenesis: Roles of Physicochemical Factors, Collagen Cleavage, and Exogenous Molecules.

Authors:  Xuening Chen; Lichen Wang; Kaitao Zhao; Hongjun Wang
Journal:  Tissue Eng Part B Rev       Date:  2018-01-05       Impact factor: 6.389

7.  Nano-hydroxyapatite modulates osteoblast lineage commitment by stimulation of DNA methylation and regulation of gene expression.

Authors:  Shin-Woo Ha; Hae Lin Jang; Ki Tae Nam; George R Beck
Journal:  Biomaterials       Date:  2015-06-23       Impact factor: 12.479

8.  HDAC5 controls MEF2C-driven sclerostin expression in osteocytes.

Authors:  Marc N Wein; Jordan Spatz; Shigeki Nishimori; John Doench; David Root; Philip Babij; Kenichi Nagano; Roland Baron; Daniel Brooks; Mary Bouxsein; Paola Divieti Pajevic; Henry M Kronenberg
Journal:  J Bone Miner Res       Date:  2015-03       Impact factor: 6.741

9.  RhoA GTPase interacts with beta-catenin signaling in clinorotated osteoblasts.

Authors:  Qiaoqiao Wan; Eunhye Cho; Hiroki Yokota; Sungsoo Na
Journal:  J Bone Miner Metab       Date:  2013-03-26       Impact factor: 2.626

Review 10.  In vitro and in vivo approaches to study osteocyte biology.

Authors:  Ivo Kalajzic; Brya G Matthews; Elena Torreggiani; Marie A Harris; Paola Divieti Pajevic; Stephen E Harris
Journal:  Bone       Date:  2012-10-13       Impact factor: 4.398

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