Literature DB >> 22130920

Rat osteoblast cultures.

Isabel R Orriss1, Sarah E B Taylor, Timothy R Arnett.   

Abstract

This chapter describes the isolation, culture and staining of primary osteoblasts from the calvaria and long bones of neonatal rats. The key advantages of this assay are that it allows direct measurement of bone matrix deposition and mineralisation, as well as yielding good quantities of osteoblasts at defined stages of differentiation for molecular and histological analysis. A special focus of this chapter is on the role of β-glycerophosphate in cell-mediated mineralisation in these cultures.

Entities:  

Mesh：

Substances：

Year:  2012        PMID： 22130920     DOI： 10.1007/978-1-61779-415-5_3

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  26 in total

1.  Generation of rodent and human osteoblasts.

Authors:  Sarah E B Taylor; Mittal Shah; Isabel R Orriss
Journal:  Bonekey Rep       Date:  2014-11-12

2.  Cystathionine γ-Lyase-Hydrogen Sulfide Induces Runt-Related Transcription Factor 2 Sulfhydration, Thereby Increasing Osteoblast Activity to Promote Bone Fracture Healing.

Authors:  Yang Zheng; Feng Liao; Xianjuan Lin; Fengjiao Zheng; Jinghui Fan; Qinghua Cui; Jichun Yang; Bin Geng; Jun Cai
Journal:  Antioxid Redox Signal       Date:  2017-03-10       Impact factor: 8.401

3.  Bone turnover is altered in transgenic rats overexpressing the P2Y2 purinergic receptor.

Authors:  Maria Ellegaard; Cansu Agca; Solveig Petersen; Ankita Agrawal; Lars Schack Kruse; Ning Wang; Alison Gartland; Jens-Erik Beck Jensen; Niklas Rye Jørgensen; Yuksel Agca
Journal:  Purinergic Signal       Date:  2017-08-21       Impact factor: 3.765

4.  Lack of effect of adenosine on the function of rodent osteoblasts and osteoclasts in vitro.

Authors:  Mark O R Hajjawi; Jessal J Patel; Michelangelo Corcelli; Timothy R Arnett; Isabel R Orriss
Journal:  Purinergic Signal       Date:  2016-02-10       Impact factor: 3.765

5.  Inhibition of arterial medial calcification and bone mineralization by extracellular nucleotides: The same functional effect mediated by different cellular mechanisms.

Authors:  Jessal J Patel; Dongxing Zhu; Britt Opdebeeck; Patrick D'Haese; José L Millán; Lucie E Bourne; Caroline P D Wheeler-Jones; Timothy R Arnett; Vicky E MacRae; Isabel R Orriss
Journal:  J Cell Physiol       Date:  2017-10-04       Impact factor: 6.384

6.  The P2X7 Receptor is an Important Regulator of Extracellular ATP Levels.

Authors:  Andrea Brandao-Burch; Michelle L Key; Jessal J Patel; Timothy R Arnett; Isabel R Orriss
Journal:  Front Endocrinol (Lausanne)       Date:  2012-03-19       Impact factor: 5.555

7.  Acidosis is a key regulator of osteoblast ecto-nucleotidase pyrophosphatase/phosphodiesterase 1 (NPP1) expression and activity.

Authors:  Isabel R Orriss; Michelle L Key; Mark O R Hajjawi; José L Millán; Timothy R Arnett
Journal:  J Cell Physiol       Date:  2015-12       Impact factor: 6.384

8.  Osteogenic potential of punica granatum through matrix mineralization, cell cycle progression and runx2 gene expression in primary rat osteoblasts.

Authors:  Sahabjada Siddiqui; Mohammad Arshad
Journal:  Daru       Date:  2014-11-20       Impact factor: 3.117

9.  Extracellular ATP released by osteoblasts is a key local inhibitor of bone mineralisation.

Authors:  Isabel R Orriss; Michelle L Key; Mark O R Hajjawi; Timothy R Arnett
Journal:  PLoS One       Date:  2013-07-09       Impact factor: 3.240

10.  Optimisation of the differing conditions required for bone formation in vitro by primary osteoblasts from mice and rats.

Authors:  Isabel R Orriss; Mark O R Hajjawi; Carmen Huesa; Vicky E MacRae; Timothy R Arnett
Journal:  Int J Mol Med       Date:  2014-09-08       Impact factor: 4.101
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.