| Literature DB >> 22129199 |
Shinsuke Ohnuki1, Tomohide Kobayashi, Hayato Ogawa, Ikuko Kozone, Jun-Ya Ueda, Motoki Takagi, Kazuo Shin-Ya, Dai Hirata, Satoru Nogami, Yoshikazu Ohya.
Abstract
To investigate the biological activity of a novel 24-membered macrolide compound, JBIR-19, isolated from the culture broth of the entomopathogenic fungus Metarhizium sp. fE61, morphological changes in yeast cells were examined using the automated image-processing program CalMorph. Principal components analysis was used to elucidate dynamic changes in the phenotypes, revealing two independent effects of JBIR-19 in yeast cells: bud elongation and increased size of the actin region. Using a fitness assay, we identified the genes required for robust growth in the presence of JBIR-19. Among these were CCW12, YLR111W, and DHH1, which are also involved in abnormal bud morphology. Based on these results and others, we predict intracellular targets of JBIR-19 and its functional interactions.Entities:
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Year: 2012 PMID： 22129199 DOI： 10.1111/j.1567-1364.2011.00770.x
Source DB: PubMed Journal: FEMS Yeast Res ISSN： 1567-1356 Impact factor: 2.796