PURPOSE: Active metabolites of arachidonic acid (AA), eicosanoids, are exerting a significant influence on renal homeostasis. In our recent paper, we demonstrated that high levels of lipoxygenase (LOX)-derived AA metabolites, hydroxyeicosatetraenoic acids (HETEs), unfavorably influence post-transplant function of kidney allografts. Hence, in this study, we wanted (1) to analyze 5-, 12- and 15-HETE levels in non-diabetic chronic kidney disease (CKD) patients, already undergoing regular hemodialysis treatment, and determine factors that may influence these eicosanoids' generation, as well as, (2) to verify whether application of glucose-containing, instead of glucose-free, dialyzing fluids may be beneficial for the limitation of 5-, 12- and 15-HETE synthesis during a single hemodialysis session. METHODS: Twenty-four healthy individuals and 50 CKD patients undergoing regular hemodialysis treatment were included in the study. CKD patients were divided into two subgroups depending on presence/absence of glucose in dialyzing fluid. LOX-derived HETEs were measured using liquid chromatography. RESULTS: Results demonstrated higher levels of examined eicosanoids in CKD patients (P<0.05 for all). Older age and higher C-reactive protein levels were associated with HETEs concentrations. Presence of glucose in dialyzing fluid significantly diminished the increase in 5- and 12-HETE synthesis (411.24% vs. 107.29%, P<0.006; 301.70% vs. 98.21%, P<0.0008, respectively), however, it did not influence 15-HETE generation (156.98% vs. 135.24%, P=0.26). CONCLUSIONS: Non-diabetic CKD patients have higher levels of LOX-derived HETEs, which are associated mainly with age and intensified inflammatory process. The presence of glucose in the dialysate is associated with a reduced synthesis of selected LOX-derived HETEs in these patients; hence, we speculate that the application of such a simple hemodialysis fluid modification may have a favorable influence on post-transplant outcomes.
PURPOSE: Active metabolites of arachidonic acid (AA), eicosanoids, are exerting a significant influence on renal homeostasis. In our recent paper, we demonstrated that high levels of lipoxygenase (LOX)-derived AA metabolites, hydroxyeicosatetraenoic acids (HETEs), unfavorably influence post-transplant function of kidney allografts. Hence, in this study, we wanted (1) to analyze 5-, 12- and 15-HETE levels in non-diabetic chronic kidney disease (CKD) patients, already undergoing regular hemodialysis treatment, and determine factors that may influence these eicosanoids' generation, as well as, (2) to verify whether application of glucose-containing, instead of glucose-free, dialyzing fluids may be beneficial for the limitation of 5-, 12- and 15-HETE synthesis during a single hemodialysis session. METHODS: Twenty-four healthy individuals and 50 CKDpatients undergoing regular hemodialysis treatment were included in the study. CKDpatients were divided into two subgroups depending on presence/absence of glucose in dialyzing fluid. LOX-derived HETEs were measured using liquid chromatography. RESULTS: Results demonstrated higher levels of examined eicosanoids in CKDpatients (P<0.05 for all). Older age and higher C-reactive protein levels were associated with HETEs concentrations. Presence of glucose in dialyzing fluid significantly diminished the increase in 5- and 12-HETE synthesis (411.24% vs. 107.29%, P<0.006; 301.70% vs. 98.21%, P<0.0008, respectively), however, it did not influence 15-HETE generation (156.98% vs. 135.24%, P=0.26). CONCLUSIONS:Non-diabetic CKDpatients have higher levels of LOX-derived HETEs, which are associated mainly with age and intensified inflammatory process. The presence of glucose in the dialysate is associated with a reduced synthesis of selected LOX-derived HETEs in these patients; hence, we speculate that the application of such a simple hemodialysis fluid modification may have a favorable influence on post-transplant outcomes.
Authors: Barbara Dolegowska; Wojciech Blogowski; Krzysztof Safranow; Leszek Domanski; Katarzyna Jakubowska; Maria Olszewska Journal: Nephrol Dial Transplant Date: 2010-06-08 Impact factor: 5.992
Authors: J P Cristol; M F Maggi; J Y Bosc; S Badiou; M Delage; M H Vernet; F Michel; J Castel; B Canaud; B Descomps Journal: C R Seances Soc Biol Fil Date: 1997
Authors: J Shen; E Herderick; J F Cornhill; E Zsigmond; H S Kim; H Kühn; N V Guevara; L Chan Journal: J Clin Invest Date: 1996-11-15 Impact factor: 14.808
Authors: M Taccone-Gallucci; S Manca-di-Villahermosa; L Battistini; R G Stuffler; M Tedesco; M Maccarrone Journal: Kidney Int Date: 2006-04 Impact factor: 10.612
Authors: Ada W Y Chung; H H Clarice Yang; Jong Moo Kim; Mhairi K Sigrist; Elliott Chum; William A Gourlay; Adeera Levin Journal: Circulation Date: 2009-08-17 Impact factor: 29.690
Authors: B Dolegowska; J Stepniewska; K Ciechanowski; K Safranow; B Millo; J Bober; D Chlubek Journal: Blood Purif Date: 2007-10-15 Impact factor: 2.614
Authors: Xinping Liu; Sung Ho Moon; David J Mancuso; Christopher M Jenkins; Shaoping Guan; Harold F Sims; Richard W Gross Journal: Anal Biochem Date: 2013-07-11 Impact factor: 3.365