Literature DB >> 2212685

Bioassay of interleukin-1 in serum and plasma following removal of inhibitory activity with polyethylene glycol.

S J Hopkins1, M Humphreys.   

Abstract

Serum and plasma normally inhibit the responsiveness of the indicator cells used in bioassays for interleukin-1 (IL-1). A precipitation step with 12% polyethylene glycol (PEG) was found to remove material responsible for such inhibition. The IL-1 bioassay could be performed in the presence of a 3% concentration of PEG and recovery of added natural IL-1 from plasma or serum was essentially complete.

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Year:  1990        PMID： 2212685     DOI： 10.1016/0022-1759(90)90326-q

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  5 in total

Review 1.  Cytokines and cytokine measurements in a clinical laboratory.

Authors:  T L Whiteside
Journal:  Clin Diagn Lab Immunol       Date:  1994-05

2.  Long-term intracerebroventricular infusion of corticotropin-releasing hormone alters neuroendocrine, neurochemical, autonomic, behavioral, and cytokine responses to a systemic inflammatory challenge.

Authors:  A C Linthorst; C Flachskamm; S J Hopkins; M E Hoadley; M S Labeur; F Holsboer; J M Reul
Journal:  J Neurosci       Date:  1997-06-01       Impact factor: 6.167

3.  Role of endogenous interleukin-1 receptor antagonist in regulating fever induced by localised inflammation in the rat.

Authors:  T Cartmell; G N Luheshi; S J Hopkins; N J Rothwell; S Poole
Journal:  J Physiol       Date:  2001-02-15       Impact factor: 5.182

Review 4.  Cytokine measurements and interpretation of cytokine assays in human disease.

Authors:  T L Whiteside
Journal:  J Clin Immunol       Date:  1994-11       Impact factor: 8.317

5.  Serum corticosterone, interleukin-1 and tumour necrosis factor in rat experimental endotoxaemia: comparison between Lewis and Wistar strains.

Authors:  M Perretti; G S Duncan; R J Flower; S H Peers
Journal:  Br J Pharmacol       Date:  1993-10       Impact factor: 8.739
  5 in total

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