Literature DB >> 2212680

Statistical design of ELISA protocols.

D S Bunch1, D M Rocke, R O Harrison.   

Abstract

This paper shows how to obtain accuracy and efficiency in an ELISA analysis by allocating the wells on a 96-well microplate between calibration and determination of unknowns, and by choosing the known concentrations for calibration. The method also can determine how much is lost in precision by using a convenient but non-optimal protocol.

Mesh：

Year:  1990        PMID： 2212680     DOI： 10.1016/0022-1759(90)90036-u

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  3 in total

1.  Concordance of bacterial cultures with endotoxin and interleukin-6 in necrotizing enterocolitis.

Authors:  L C Duffy; M A Zielezny; V Carrion; E Griffiths; D Dryja; M Hilty; C Rook; F Morin
Journal:  Dig Dis Sci       Date:  1997-02       Impact factor: 3.199

2.  Expression of recombinant human serum amyloid A in mammalian cells and demonstration of the region necessary for high-density lipoprotein binding and amyloid fibril formation by site-directed mutagenesis.

Authors:  H Patel; J Bramall; H Waters; M C De Beer; P Woo
Journal:  Biochem J       Date:  1996-09-15       Impact factor: 3.857

3.  Evaluating concentration estimation errors in ELISA microarray experiments.

Authors:  Don Simone Daly; Amanda M White; Susan M Varnum; Kevin K Anderson; Richard C Zangar
Journal:  BMC Bioinformatics       Date:  2005-01-26       Impact factor: 3.169
  3 in total

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