Literature DB >> 2212623

An improved staining method for rat microglial cells using the lectin from Griffonia simplicifolia (GSA I-B4).

W J Streit1.   

Abstract

A simple method for the lectin histochemical visualization of rat microglial cells is described. Advantages include ease of fixation of brain tissue using paraformaldehyde, and rapidity of tissue processing by vibratome sectioning. Furthermore, in addition to providing good structural preservation, the method achieves improved lectin binding, resulting in complete labeling of all microglial cells and in superior visualization of cellular processes. The lectin histochemical technique for rat microglia has the potential to be adapted to any mammalian species, and should prove valuable for neuroscientists interested in studying this glial cell type.

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Year:  1990        PMID： 2212623     DOI： 10.1177/38.11.2212623

Source DB:  PubMed          Journal:  J Histochem Cytochem        ISSN： 0022-1554            Impact factor:   2.479


  62 in total

1.  Chronic exposure to corticosterone enhances the neuroinflammatory and neurotoxic responses to methamphetamine.

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2.  In vivo molecular markers for pro-inflammatory cytokine M1 stage and resident microglia in trimethyltin-induced hippocampal injury.

Authors:  C A McPherson; B A Merrick; G J Harry
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3.  Differential expression of class 3 and 4 semaphorins and netrin in the lamprey spinal cord during regeneration.

Authors:  Michael I Shifman; Michael E Selzer
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4.  D-serine is a key determinant of glutamate toxicity in amyotrophic lateral sclerosis.

Authors:  Jumpei Sasabe; Tomohiro Chiba; Marina Yamada; Koichi Okamoto; Ikuo Nishimoto; Masaaki Matsuoka; Sadakazu Aiso
Journal:  EMBO J       Date:  2007-08-30       Impact factor: 11.598

5.  Ethanol preconditioning protects against ischemia/reperfusion-induced brain damage: role of NADPH oxidase-derived ROS.

Authors:  Qun Wang; Albert Y Sun; Agnes Simonyi; Theodore J Kalogeris; Dennis K Miller; Grace Y Sun; Ronald J Korthuis
Journal:  Free Radic Biol Med       Date:  2007-07-04       Impact factor: 7.376

6.  Microglial activation in the hippocampus of hypercholesterolemic rabbits occurs independent of increased amyloid production.

Authors:  Qing-Shan Xue; D Larry Sparks; Wolfgang J Streit
Journal:  J Neuroinflammation       Date:  2007-08-24       Impact factor: 8.322

7.  Role for neuronally derived fractalkine in mediating interactions between neurons and CX3CR1-expressing microglia.

Authors:  J K Harrison; Y Jiang; S Chen; Y Xia; D Maciejewski; R K McNamara; W J Streit; M N Salafranca; S Adhikari; D A Thompson; P Botti; K B Bacon; L Feng
Journal:  Proc Natl Acad Sci U S A       Date:  1998-09-01       Impact factor: 11.205

8.  Induction of Hsp27 and Hsp32 stress proteins and vimentin in glial cells of the rat hippocampus following hyperthermia.

Authors:  David A Bechtold; Ian R Brown
Journal:  Neurochem Res       Date:  2003-08       Impact factor: 3.996

9.  Dopamine disposition in the presynaptic process regulates the severity of methamphetamine-induced neurotoxicity.

Authors:  Donald M Kuhn; Dina M Francescutti-Verbeem; David M Thomas
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10.  Induction of nitric oxide synthase and microglial responses precede selective cell death induced by chronic impairment of oxidative metabolism.

Authors:  N Y Calingasan; L C Park; L L Calo; R R Trifiletti; S E Gandy; G E Gibson
Journal:  Am J Pathol       Date:  1998-08       Impact factor: 4.307
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