OBJECTIVES: This study aims to study how Bio-Oss® can induce osteoblast differentiation in mesenchymal stem cells, the expression levels of bone related genes and mesenchymal stem cells markers using real time Reverse Transcription-Polymerase Chain Reaction. METHODS: PB-hMSCs stem preparations were obtained for gradient centrifugation from peripheral blood of healthy anonymous volunteers, using the Acuspin System-Histopaque 1077. The samples were then cultured for 7 days for RNA processing, and the expression was quantified using real time PCR. RESULTS: Bio-Oss® caused an induction of osteoblast transcriptional factor like RUNX2 and of bone related genes; SPP1 and FOSL1. In contrast, the expression of ENG was significantly decreased in stem cells treated with Bio-Oss® with respect to untreated cells, indicating the differentiation effect of this biomaterial on stem cells. CONCLUSION: The results obtained can be relevant to enhance the understanding of the molecular mechanism of bone regeneration and can act as a model for comparing other materials with similar clinical effects.
OBJECTIVES: This study aims to study how Bio-Oss® can induce osteoblast differentiation in mesenchymal stem cells, the expression levels of bone related genes and mesenchymal stem cells markers using real time Reverse Transcription-Polymerase Chain Reaction. METHODS: PB-hMSCs stem preparations were obtained for gradient centrifugation from peripheral blood of healthy anonymous volunteers, using the Acuspin System-Histopaque 1077. The samples were then cultured for 7 days for RNA processing, and the expression was quantified using real time PCR. RESULTS: Bio-Oss® caused an induction of osteoblast transcriptional factor like RUNX2 and of bone related genes; SPP1 and FOSL1. In contrast, the expression of ENG was significantly decreased in stem cells treated with Bio-Oss® with respect to untreated cells, indicating the differentiation effect of this biomaterial on stem cells. CONCLUSION: The results obtained can be relevant to enhance the understanding of the molecular mechanism of bone regeneration and can act as a model for comparing other materials with similar clinical effects.
Authors: S Pappalardo; M Coronella; M L Lanza; V Rabbito; P V Foti; L A Mauro; S Palmucci; G C Ettorre Journal: Radiol Med Date: 2013-01-28 Impact factor: 3.469
Authors: Marcelo de Oliveira E Silva; André Antonio Pelegrine; Alexandre Alves Pinheiro da Silva; Luiz Roberto Manhães Júnior; Rafael de Mello E Oliveira; Silvana Gaiba França; Antonio Carlos Aloise; Lydia Masako Ferreira Journal: Int J Biomater Date: 2012-08-29
Authors: Stefania Lymperi; Vasiliki Taraslia; Ioannis N Tsatsoulis; Athina Samara; Athanasios D Velentzas; Anastasia Agrafioti; Ema Anastasiadou; Evangelos Kontakiotis Journal: Biomed Res Int Date: 2015-06-03 Impact factor: 3.411
Authors: Paulo José Pasquali; Marcelo Lucchesi Teixeira; Thiago Altro de Oliveira; Luis Guilherme Scavone de Macedo; Antonio Carlos Aloise; André Antonio Pelegrine Journal: Int J Biomater Date: 2015-10-12