Literature DB >> 22124943

Mechanism-oriented redesign of an isomaltulose synthase to an isomelezitose synthase by site-directed mutagenesis.

Julian Görl1, Malte Timm, Jürgen Seibel.   

Abstract

An isomelezitose synthase was redesigned out of the sucrose isomerase from Protaminobacter rubrum for the synthesis of isomelezitose (6-O(F)-glucosylsucrose), a potential nutraceutical. The variants F297A, F297P, R333K, F321A_F319A and E428D catalyze the formation of isomelezitose in up to 70 % yield.
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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Year:  2011        PMID： 22124943     DOI： 10.1002/cbic.201100576

Source DB:  PubMed          Journal:  Chembiochem        ISSN： 1439-4227            Impact factor:   3.164


  5 in total

1.  Toward Understanding the Catalytic Mechanism of Human Paraoxonase 1: Site-Specific Mutagenesis at Position 192.

Authors:  Geetika Aggarwal; Rameshwar Prajapati; Rajan K Tripathy; Priyanka Bajaj; A R Satvik Iyengar; Abhay T Sangamwar; Abhay H Pande
Journal:  PLoS One       Date:  2016-02-01       Impact factor: 3.240

2.  Efficient production of isomelezitose by a glucosyltransferase activity in Metschnikowia reukaufii cell extracts.

Authors:  Martin Garcia-Gonzalez; Francisco J Plou; Fadia V Cervantes; Miguel Remacha; Ana Poveda; Jesús Jiménez-Barbero; Maria Fernandez-Lobato
Journal:  Microb Biotechnol       Date:  2019-10-01       Impact factor: 5.813

3.  The structural basis of Erwinia rhapontici isomaltulose synthase.

Authors:  Zheng Xu; Sha Li; Jie Li; Yan Li; Xiaohai Feng; Renxiao Wang; Hong Xu; Jiahai Zhou
Journal:  PLoS One       Date:  2013-09-19       Impact factor: 3.240

4.  Molecular characterization and heterologous expression of two α-glucosidases from Metschnikowia spp, both producers of honey sugars.

Authors:  Martin Garcia-Gonzalez; Marina Minguet-Lobato; Francisco J Plou; Maria Fernandez-Lobato
Journal:  Microb Cell Fact       Date:  2020-07-11       Impact factor: 5.328

5.  Engineering a Highly Active Sucrose Isomerase for Enhanced Product Specificity by Using a "Battleship" Strategy.

Authors:  Patrick Pilak; André Schiefner; Judith Seiboth; Johannes Oehrlein; Arne Skerra
Journal:  Chembiochem       Date:  2020-04-16       Impact factor: 3.164
  5 in total

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