Literature DB >> 22123058

Generation of scFv phages specific to Staphylococcus enterotoxin C1 by panning on related antigens.

Anna V Lomonosova1, Alexander G Laman, Ksenia K Fursova, Anna O Shepelyakovskaya, Yury V Vertiev, Fedor A Brovko, Evgeny V Grishin.   

Abstract

A method for generation of highly specific miniantibodies within the phage particle has been developed, and used to produce antibodies against Staphylococcus enterotoxin type C1. Under successive panning of the non-immune phage miniantibody (scFv) library with enterotoxins SE (types A, B, C1, D, E, G, and I) adsorbed on the plate surface, we generated 11 individual phage clones to Staphylococcus enterotoxin type C1. Five of them interacted specifically only with SEC1 and had no cross-reactions with the other enterotoxins.

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Year:  2011        PMID： 22123058      PMCID： PMC3242837          DOI： 10.4161/mabs.3.6.18089

Source DB:  PubMed          Journal:  MAbs        ISSN： 1942-0862            Impact factor:   5.857


  8 in total

1.  Simultaneous detection of seven staphylococcal enterotoxins: development of hydrogel biochips for analytical and practical application.

Authors:  A Yu Rubina; M A Filippova; G U Feizkhanova; A O Shepeliakovskaya; E I Sidina; Kh M Boziev; A G Laman; F A Brovko; Yu V Vertiev; A S Zasedatelev; E V Grishin
Journal:  Anal Chem       Date:  2010-09-15       Impact factor: 6.986

Review 2.  Selecting and screening recombinant antibody libraries.

Authors:  Hennie R Hoogenboom
Journal:  Nat Biotechnol       Date:  2005-09       Impact factor: 54.908

Review 3.  Applications of single-chain variable fragment antibodies in therapeutics and diagnostics.

Authors:  Nina E Weisser; J Christopher Hall
Journal:  Biotechnol Adv       Date:  2009-04-15       Impact factor: 14.227

4.  The isolation of scFvs against small target molecules.

Authors:  Iain Shaw; Marian Kane
Journal:  Methods Mol Biol       Date:  2009

5.  Cross-reactive HIV-1 neutralizing monoclonal antibodies selected by screening of an immune human phage library against an envelope glycoprotein (gp140) isolated from a patient (R2) with broadly HIV-1 neutralizing antibodies.

Authors:  Vidita Choudhry; Mei-Yun Zhang; Igor A Sidorov; John M Louis; Ilia Harris; Antony S Dimitrov; Peter Bouma; Fatim Cham; Anil Choudhary; Susanna M Rybak; Timothy Fouts; David C Montefiori; Christopher C Broder; Gerald V Quinnan; Dimiter S Dimitrov
Journal:  Virology       Date:  2007-02-15       Impact factor: 3.616

6.  The remarkable flexibility of the human antibody repertoire; isolation of over one thousand different antibodies to a single protein, BLyS.

Authors:  Bryan M Edwards; Steven C Barash; Sarah H Main; Gil H Choi; Ralph Minter; Stephen Ullrich; Elizabeth Williams; Leila Du Fou; Jane Wilton; Vivian R Albert; Steve M Ruben; Tristan J Vaughan
Journal:  J Mol Biol       Date:  2003-11-14       Impact factor: 5.469

7.  [Molecular cloning, expression, and characterization of human mini-antibodies against enterotoxin C1 of Staphylococcus aureus].

Authors:  N A Kalinina; L V Samokhvalova; A B Ulitin; Iu V Vertiev; V A Nesmeianov
Journal:  Bioorg Khim       Date:  2009 Mar-Apr

Review 8.  Diversity in Staphylococcus aureus enterotoxins.

Authors:  Damien Thomas; Seemay Chou; Olivier Dauwalder; Gerard Lina
Journal:  Chem Immunol Allergy       Date:  2007
  8 in total
  1 in total

Review 1.  Phage display--a powerful technique for immunotherapy: 1. Introduction and potential of therapeutic applications.

Authors:  Justyna Bazan; Ireneusz Całkosiński; Andrzej Gamian
Journal:  Hum Vaccin Immunother       Date:  2012-08-21       Impact factor: 3.452
  1 in total

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