BACKGROUND: Cervical cancer (CC) is a common malignancy in women worldwide. Cervical tumorigenesis involves a multistep process in which accumulations of genetic alterations are present. Homeotic genes, such as HOX gene re-expression, have been reported in a wide variety of tumors. METHODS: In order to know the role of HOX B4 gene expression in CC, in the present study, two-dimensional polyacrylamide gel electrophoresis, matrix-assisted laser desorption/ionization, and time-of-flight mass spectrometry were used for differential screening of protein expression in CC. Immunohistochemical analysis was performed on the cervical tissue microarray (TMA) to detect the Hox B4 protein. RESULTS: Hox B4 peptide was detected among 15 increased spots differentially observed in CC. Using TMA, Hox B4 protein was also immunodetected in the nuclei of cervical epithelial tumor cells, while in normal cervical epithelium, it was absent. Interestingly, it was possible to detect the Hox B4 protein in the precursor lesions. CONCLUSIONS: Hox B4 protein is present in the precursor lesions as CC cells, suggesting that Hox B4 could be a protein related to the neoplastic state (non-differentiated cells) of human cervical epithelium.
BACKGROUND: Cervical cancer (CC) is a common malignancy in women worldwide. Cervical tumorigenesis involves a multistep process in which accumulations of genetic alterations are present. Homeotic genes, such as HOX gene re-expression, have been reported in a wide variety of tumors. METHODS: In order to know the role of HOX B4 gene expression in CC, in the present study, two-dimensional polyacrylamide gel electrophoresis, matrix-assisted laser desorption/ionization, and time-of-flight mass spectrometry were used for differential screening of protein expression in CC. Immunohistochemical analysis was performed on the cervical tissue microarray (TMA) to detect the Hox B4 protein. RESULTS:Hox B4 peptide was detected among 15 increased spots differentially observed in CC. Using TMA, Hox B4 protein was also immunodetected in the nuclei of cervical epithelial tumor cells, while in normal cervical epithelium, it was absent. Interestingly, it was possible to detect the Hox B4 protein in the precursor lesions. CONCLUSIONS:Hox B4 protein is present in the precursor lesions as CC cells, suggesting that Hox B4 could be a protein related to the neoplastic state (non-differentiated cells) of human cervical epithelium.
Authors: Emanuel F Petricoin; Ali M Ardekani; Ben A Hitt; Peter J Levine; Vincent A Fusaro; Seth M Steinberg; Gordon B Mills; Charles Simone; David A Fishman; Elise C Kohn; Lance A Liotta Journal: Lancet Date: 2002-02-16 Impact factor: 79.321
Authors: Elwin A Morgan; Shiva S Forootan; Janet Adamson; Christopher S Foster; Hiroshi Fujii; Michihiro Igarashi; Carol Beesley; Paul H Smith; Youqiang Ke Journal: Int J Oncol Date: 2008-04 Impact factor: 5.650
Authors: Monica Cantile; Renato Franco; Adrienne Tschan; Daniel Baumhoer; Inti Zlobec; Giulia Schiavo; Iris Forte; Michel Bihl; Giuseppina Liguori; Gerardo Botti; Luigi Tornillo; Eva Karamitopoulou-Diamantis; Luigi Terracciano; Clemente Cillo Journal: Int J Cancer Date: 2009-10-01 Impact factor: 7.396
Authors: Li-Jun Tang; Francesco De Seta; Federico Odreman; Per Venge; Caterina Piva; Secondo Guaschino; Rodolfo C Garcia Journal: J Proteome Res Date: 2007-06-01 Impact factor: 4.466
Authors: Ye Gu; Shiaw-Lin Wu; Jane L Meyer; William S Hancock; Lawrence J Burg; James Linder; David W Hanlon; Barry L Karger Journal: J Proteome Res Date: 2007-09-29 Impact factor: 4.466
Authors: Steffen Jørgensen; Mehmet Coskun; Keld Mikkelsen Homburg; Ole B V Pedersen; Jesper T Troelsen Journal: PLoS One Date: 2016-10-18 Impact factor: 3.240