| Literature DB >> 22116162 |
Vanessa Zubach1, Gerry Smart, Samuel Ratnam, Alberto Severini.
Abstract
We have developed a novel microsphere-based genotyping method for 46 mucosal human papillomavirus (HPV) types. HPV DNA was amplified by PCR using general primers and typed by hybridization to HPV type-specific probes coupled to sortable microspheres based on the Luminex xMAP technology. Hybridization to each probe was specific for each HPV type without cross-hybridization and sensitive enough to allow typing of HPV contained in clinical specimens. The method was validated with direct sequencing and the Roche Linear Array genotyping method.Entities:
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Year: 2011 PMID: 22116162 PMCID: PMC3264170 DOI: 10.1128/JCM.06090-11
Source DB: PubMed Journal: J Clin Microbiol ISSN: 0095-1137 Impact factor: 5.948