Literature DB >> 22114257

A simple method for the immunocytochemical detection of proteins inside nuclear structures that are inaccessible to specific antibodies.

Darya M Svistunova1, Yana R Musinova, Vladimir Yu Polyakov, Eugene V Sheval.   

Abstract

It has been demonstrated elsewhere that a high concentration of an antigen within the nucleolus may prevent its proper recognition by specific antibodies. In this study, the authors found that a short proteinase treatment allowed for the detection of antigens in the nucleoli. The described approach is compatible with the simultaneous observation of proteins fused to fluorescent tags and with preembedding electron microscopy. It appears that the described method can be useful in situations when the proper recognition of antigens by specific antibodies is disturbed by a high density of cellular structures or a high concentration of antigens inside these structures.

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Year:  2011        PMID： 22114257      PMCID： PMC3351117          DOI： 10.1369/0022155411429704

Source DB:  PubMed          Journal:  J Histochem Cytochem        ISSN： 0022-1554            Impact factor:   2.479


  19 in total

1.  A two-step scaffolding model for mitotic chromosome assembly.

Authors:  Kazuhiro Maeshima; Ulrich K Laemmli
Journal:  Dev Cell       Date:  2003-04       Impact factor: 12.270

2.  Chromosome structure: improved immunolabeling for electron microscopy.

Authors:  Kazuhiro Maeshima; Michail Eltsov; Ulrich K Laemmli
Journal:  Chromosoma       Date:  2005-11-12       Impact factor: 4.316

3.  A higher concentration of an antigen within the nucleolus may prevent its proper recognition by specific antibodies.

Authors:  E V Sheval; M A Polzikov; M O J Olson; O V Zatsepina
Journal:  Eur J Histochem       Date:  2005 Apr-Jun       Impact factor: 3.188

4.  Visualization of the chromosome scaffold and intermediates of loop domain compaction in extracted mitotic cells.

Authors:  Eugene V Sheval; Vladimir Y Polyakov
Journal:  Cell Biol Int       Date:  2006-08-24       Impact factor: 3.612

5.  Cell cycle-dependent translocations of a major nucleolar phosphoprotein, B23, and some characteristics of its variants.

Authors:  O V Zatsepina; I T Todorov; R N Philipova; C P Krachmarov; M F Trendelenburg; E G Jordan
Journal:  Eur J Cell Biol       Date:  1997-05       Impact factor: 4.492

6.  Simultaneous immunoelectron microscopic visualization of protein B23 and C23 distribution in the HeLa cell nucleolus.

Authors:  M Biggiogera; S Fakan; S H Kaufmann; A Black; J H Shaper; H Busch
Journal:  J Histochem Cytochem       Date:  1989-09       Impact factor: 2.479

7.  Temporal and spatial control of nucleophosmin by the Ran-Crm1 complex in centrosome duplication.

Authors:  Wei Wang; Anuradha Budhu; Marshonna Forgues; Xin Wei Wang
Journal:  Nat Cell Biol       Date:  2005-07-24       Impact factor: 28.824

8.  Glycol methacrylate (GMA) embedding for light microscopy. II. Immunohistochemical analysis of semithin sections of undecalcified marrow cores.

Authors:  A Islam; E Archimbaud; E S Henderson; T Han
Journal:  J Clin Pathol       Date:  1988-08       Impact factor: 3.411

9.  Immunohistochemistry of germ cell and trophoblastic neoplasms.

Authors:  G A Niehans; J C Manivel; G T Copland; B W Scheithauer; M R Wick
Journal:  Cancer       Date:  1988-09-15       Impact factor: 6.860

10.  The use of electron microscopic immunocytochemistry with silver-enhanced 1.4-nm gold particles to localize GAD in the cerebellar nuclei.

Authors:  H G Gilerovitch; G A Bishop; J S King; R W Burry
Journal:  J Histochem Cytochem       Date:  1995-03       Impact factor: 2.479
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  8 in total

1.  Identification and characterization of nuclear and nucleolar localization signals in the adeno-associated virus serotype 2 assembly-activating protein.

Authors:  Lauriel F Earley; Yasuhiro Kawano; Kei Adachi; Xiao-Xin Sun; Mu-Shui Dai; Hiroyuki Nakai
Journal:  J Virol       Date:  2014-12-31       Impact factor: 5.103

2.  Analysis of K-Ras Interactions by Biotin Ligase Tagging.

Authors:  Christopher Ritchie; Andrew Mack; Logan Harper; Ayna Alfadhli; Philip J S Stork; Xiaolin Nan; Eric Barklis
Journal:  Cancer Genomics Proteomics       Date:  2017 Jul-Aug       Impact factor: 4.069

Review 3.  Reply to Sheval, E.V. Comment on "Piña et al. Ten Approaches That Improve Immunostaining: A Review of the Latest Advances for the Optimization of Immunofluorescence. Int. J. Mol. Sci. 2022, 23, 1426".

Authors:  Ricardo Piña; Isabel Acosta-Galeana; Abraham Rosas-Arellano
Journal:  Int J Mol Sci       Date:  2022-04-15       Impact factor: 6.208

Review 4.  Recent Investigations on Neurotransmitters' Role in Acute White Matter Injury of Perinatal Glia and Pharmacotherapies-Glia Dynamics in Stem Cell Therapy.

Authors:  Narasimha M Beeraka; P R Hemanth Vikram; M V Greeshma; Chinnappa A Uthaiah; Tahani Huria; Junqi Liu; Pramod Kumar; Vladimir N Nikolenko; Kirill V Bulygin; Mikhail Y Sinelnikov; Olga Sukocheva; Ruitai Fan
Journal:  Mol Neurobiol       Date:  2022-01-18       Impact factor: 5.590

Review 5.  Ten Approaches That Improve Immunostaining: A Review of the Latest Advances for the Optimization of Immunofluorescence.

Authors:  Ricardo Piña; Alma I Santos-Díaz; Erika Orta-Salazar; Azucena Ruth Aguilar-Vazquez; Carola A Mantellero; Isabel Acosta-Galeana; Argel Estrada-Mondragon; Mara Prior-Gonzalez; Jadir Isai Martinez-Cruz; Abraham Rosas-Arellano
Journal:  Int J Mol Sci       Date:  2022-01-26       Impact factor: 5.923

6.  Comment on Piña et al. Ten Approaches That Improve Immunostaining: A Review of the Latest Advances for the Optimization of Immunofluorescence. Int. J. Mol. Sci. 2022, 23, 1426.

Authors:  Eugene V Sheval
Journal:  Int J Mol Sci       Date:  2022-04-15       Impact factor: 5.923

7.  Mapping the nucleolar proteome reveals a spatiotemporal organization related to intrinsic protein disorder.

Authors:  Lovisa Stenström; Diana Mahdessian; Christian Gnann; Anthony J Cesnik; Wei Ouyang; Manuel D Leonetti; Mathias Uhlén; Sara Cuylen-Haering; Peter J Thul; Emma Lundberg
Journal:  Mol Syst Biol       Date:  2020-08       Impact factor: 11.429

8.  Translocation of chromatin proteins to nucleoli-The influence of protein dynamics on post-fixation localization.

Authors:  Mirosław Zarębski; Rosevalentine Bosire; Julita Wesołowska; Oskar Szelest; Ahmed Eatmann; Katarzyna Jasińska-Konior; Oliver Kepp; Guido Kroemer; Gabor Szabo; Jurek W Dobrucki
Journal:  Cytometry A       Date:  2021-06-10       Impact factor: 4.714
  8 in total

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