Jing Guo1, Li-Jie Liu, Li Yuan, Ning Wang, Wei De. 1. Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
Abstract
AIM: To investigate the expression and localization of paxillin in rat pancreas during development. METHODS: Pancreata from Sprague Dawley rat fetuses, embryos, young animals, and adult animals were used in this study. Expression levels of paxillin in pancreata of different development stages were detected by reverse transcription polymerase chain reaction and Western blotting. To identify the cell location of paxillin in the developing rat pancreas, immunohistochemistry and double-immunofluorescent staining were performed using antibodies for specific cell markers and paxillin, respectively. RESULTS: The highest paxillin mRNA level was detected at E15.5 (embryo day 15.5) following a decrease in the later developmental periods (P < 0.05 vs E18.5, P0 and adult, respectively), and a progressively increased paxillin protein expression through the transition from E15.5 to adult was detected. The paxillin positive staining was mainly localized in rat islets of Langerhans at each stage tested during pancreas development. CONCLUSION: The dynamic expression of paxillin in rat pancreas from different stages indicates that paxillin might be involved in some aspects of pancreatic development.
AIM: To investigate the expression and localization of paxillin in rat pancreas during development. METHODS: Pancreata from Sprague Dawley rat fetuses, embryos, young animals, and adult animals were used in this study. Expression levels of paxillin in pancreata of different development stages were detected by reverse transcription polymerase chain reaction and Western blotting. To identify the cell location of paxillin in the developing rat pancreas, immunohistochemistry and double-immunofluorescent staining were performed using antibodies for specific cell markers and paxillin, respectively. RESULTS: The highest paxillin mRNA level was detected at E15.5 (embryo day 15.5) following a decrease in the later developmental periods (P < 0.05 vs E18.5, P0 and adult, respectively), and a progressively increased paxillin protein expression through the transition from E15.5 to adult was detected. The paxillin positive staining was mainly localized in rat islets of Langerhans at each stage tested during pancreas development. CONCLUSION: The dynamic expression of paxillin in rat pancreas from different stages indicates that paxillin might be involved in some aspects of pancreatic development.
Authors: Katarzyna Malenczyk; Erik Keimpema; Fabiana Piscitelli; Daniela Calvigioni; Peyman Björklund; Kenneth Mackie; Vincenzo Di Marzo; Tomas G M Hökfelt; Agnieszka Dobrzyn; Tibor Harkany Journal: Proc Natl Acad Sci U S A Date: 2015-10-22 Impact factor: 11.205
Authors: Igor Erjavec; Tatjana Bordukalo-Niksic; Jelena Brkljacic; Danka Grcevic; Gordana Mokrovic; Maja Kesic; Dunja Rogic; William Zavadoski; Vishwas M Paralkar; Lovorka Grgurevic; Vladimir Trkulja; Lipa Cicin-Sain; Slobodan Vukicevic Journal: PLoS One Date: 2016-02-23 Impact factor: 3.240