PURPOSE: To investigate the effects of Akt/ARK5 pathways on the metastatic potential of human breast cancer cells. MATERIALS AND METHODS: The human ARK5 gene was transfected into MDA-MB-231 cells. Effects of ARK5 on MDA-MB-231 cells were investigated in vitro. The tumorigenicity and spontaneously metastatic capability regulated by ARK5 were determined using an orthotopic xenograft tumor model. RESULTS: ARK5 enhanced the invasive and metastatic potential of MDA-MB-231 cells under regulation by Akt. The enhancement was associated with increasing MMP-2, MMP-9, and MT1-MMP expression. The results were further demonstrated by RNA interference experiment. In an in vivo study, we also demonstrated that ARK5-transfected breast cancer cells grew faster and had more pulmonary metastases than its parental counterparts. CONCLUSION: ARK5 led to a more invasive phenotype and metastatic potential in human breast cancer dependent on Akt.
PURPOSE: To investigate the effects of Akt/ARK5 pathways on the metastatic potential of humanbreast cancer cells. MATERIALS AND METHODS: The humanARK5 gene was transfected into MDA-MB-231 cells. Effects of ARK5 on MDA-MB-231 cells were investigated in vitro. The tumorigenicity and spontaneously metastatic capability regulated by ARK5 were determined using an orthotopic xenograft tumor model. RESULTS:ARK5 enhanced the invasive and metastatic potential of MDA-MB-231 cells under regulation by Akt. The enhancement was associated with increasing MMP-2, MMP-9, and MT1-MMP expression. The results were further demonstrated by RNA interference experiment. In an in vivo study, we also demonstrated that ARK5-transfected breast cancer cells grew faster and had more pulmonary metastases than its parental counterparts. CONCLUSION:ARK5 led to a more invasive phenotype and metastatic potential in humanbreast cancer dependent on Akt.
Authors: Xin-Zhong Chang; Jie Yu; Xue-Hui Zhang; Jian Yin; Tao Wang; Xu-Chen Cao Journal: J Cancer Res Clin Oncol Date: 2008-10-10 Impact factor: 4.553
Authors: Rachel E Bell; Mehdi Khaled; Dvir Netanely; Steffen Schubert; Tamar Golan; Amir Buxbaum; Maja M Janas; Benny Postolsky; Michael S Goldberg; Ron Shamir; Carmit Levy Journal: J Invest Dermatol Date: 2013-08-09 Impact factor: 8.551
Authors: Nathan Benaich; Samuel Woodhouse; Stephen J Goldie; Ajay Mishra; Sven R Quist; Fiona M Watt Journal: Cell Rep Date: 2014-10-02 Impact factor: 9.423