INTRODUCTION: Community effect is a phenomenon caused by cell-cell communication during myogenesis. In myogenic C2C12 cells in vitro, the confluent phase is needed for myogenesis induction. METHODS: To examine the cell-density effect, growth kinetics and myogenic differentiation were investigated in cells plated at four different cell densities. RESULTS: We found that expression of a myogenic differentiation marker was high in a density-dependent manner. At high density, where cell-cell contact was obvious, contact inhibition after the proliferation stage was accompanied by microarray findings demonstrating upregulation of negative regulating cell-cycle markers, including CDKI p21 and the muscle differentiation markers MyoD and myogenin. Interestingly, developmentally regulated protein expression (drebrin) protein expression was also upregulated in a density-dependent manner. CONCLUSIONS: These results suggest that contact inhibition after the proliferation stage may induce growth arrest via cell-cell communication through the expression of CDKI p21 and may be responsible for progressing cell fusion.
INTRODUCTION: Community effect is a phenomenon caused by cell-cell communication during myogenesis. In myogenic C2C12 cells in vitro, the confluent phase is needed for myogenesis induction. METHODS: To examine the cell-density effect, growth kinetics and myogenic differentiation were investigated in cells plated at four different cell densities. RESULTS: We found that expression of a myogenic differentiation marker was high in a density-dependent manner. At high density, where cell-cell contact was obvious, contact inhibition after the proliferation stage was accompanied by microarray findings demonstrating upregulation of negative regulating cell-cycle markers, including CDKIp21 and the muscle differentiation markers MyoD and myogenin. Interestingly, developmentally regulated protein expression (drebrin) protein expression was also upregulated in a density-dependent manner. CONCLUSIONS: These results suggest that contact inhibition after the proliferation stage may induce growth arrest via cell-cell communication through the expression of CDKIp21 and may be responsible for progressing cell fusion.
Authors: Jun Liu; Hockin H K Xu; Hongzhi Zhou; Michael D Weir; Qianming Chen; Carroll Ann Trotman Journal: Acta Biomater Date: 2012-08-16 Impact factor: 8.947
Authors: Neil I Bower; Daniel Garcia de la Serrana; Nicholas J Cole; Georgina E Hollway; Hung-Tai Lee; Stephen Assinder; Ian A Johnston Journal: J Biol Chem Date: 2012-10-17 Impact factor: 5.157
Authors: Konstantin G Lyamzaev; Alisa A Panteleeva; Anna A Karpukhina; Ivan I Galkin; Ekatherina N Popova; Olga Yu Pletjushkina; Bettina Rieger; Karin B Busch; Armen Y Mulkidjanian; Boris V Chernyak Journal: Oxid Med Cell Longev Date: 2020-02-10 Impact factor: 6.543
Authors: H Qiu; N Liu; L Luo; J Zhong; Z Tang; K Kang; J Qu; W Peng; L Liu; L Li; D Gou Journal: Cell Death Differ Date: 2016-06-17 Impact factor: 15.828
Authors: Amaresh C Panda; Kotb Abdelmohsen; Jennifer L Martindale; Clara Di Germanio; Xiaoling Yang; Ioannis Grammatikakis; Ji Heon Noh; Yongqing Zhang; Elin Lehrmann; Dawood B Dudekula; Supriyo De; Kevin G Becker; Elizabeth J White; Gerald M Wilson; Rafael de Cabo; Myriam Gorospe Journal: Nucleic Acids Res Date: 2016-01-26 Impact factor: 16.971