Akikazu Sakudo1, Yoshikazu Suganuma, Rina Sakima, Kazuyoshi Ikuta. 1. Department of Virology, Center for Infectious Disease Control, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan. sakudo@biken.osaka-u.ac.jp
Abstract
BACKGROUND: Previously, we investigated the possibility of using near-infrared (NIR) spectroscopy for the diagnosis of human immunodeficiency virus type-1 (HIV-1) infection. Here, we further analyze NIR spectra using molecular clones of various HIV-1 subtypes. METHODS: Culture supernatants of pNL4-3- (HIV-1 molecular clone) or pUC18- (empty vector) transfected 293 T cells were used. In addition, culture supernatants obtained using pBal (HIV-1 subtype B molecular clone) or pIndieC (HIV-1 subtype C molecular clone) were used. Near-infrared radiation (NIR) spectra, obtained using the culture supernatants, were subjected to principal component analysis (PCA) to extract and analyze their properties. RESULTS: The PCA demonstrated that HIV-1 in medium altered wavelength absorption at around 950 and 1030 nm, suggesting that the HIV-1 altered OH vibration in water. In addition, absorption varied among subtypes at around 950, 1030 and 1060 nm, suggesting that the interaction between HIV-1 and water varies among subtypes. CONCLUSIONS: These differences in the NIR spectra may make it possible to delineate HIV-1 subtypes spectroscopically.
BACKGROUND: Previously, we investigated the possibility of using near-infrared (NIR) spectroscopy for the diagnosis of human immunodeficiency virus type-1 (HIV-1) infection. Here, we further analyze NIR spectra using molecular clones of various HIV-1 subtypes. METHODS: Culture supernatants of pNL4-3- (HIV-1 molecular clone) or pUC18- (empty vector) transfected 293 T cells were used. In addition, culture supernatants obtained using pBal (HIV-1 subtype B molecular clone) or pIndieC (HIV-1 subtype C molecular clone) were used. Near-infrared radiation (NIR) spectra, obtained using the culture supernatants, were subjected to principal component analysis (PCA) to extract and analyze their properties. RESULTS: The PCA demonstrated that HIV-1 in medium altered wavelength absorption at around 950 and 1030 nm, suggesting that the HIV-1 altered OH vibration in water. In addition, absorption varied among subtypes at around 950, 1030 and 1060 nm, suggesting that the interaction between HIV-1 and water varies among subtypes. CONCLUSIONS: These differences in the NIR spectra may make it possible to delineate HIV-1 subtypes spectroscopically.
Authors: Daniel L D Freitas; Ana F S Peres; Lidiane G Silva; João V M Mariz; Marcos G Santos; Rayanne S P Morais; Camilo L M Morais; Francis L Martin; Daniel A V Pascoal; Juliana D de A S Camargo; Janaina C O Crispim; Kassio M G Lima Journal: Sci Rep Date: 2021-11-19 Impact factor: 4.379
Authors: Marfran C D Santos; Camilo L M Morais; Yasmin M Nascimento; Josélio M G Araujo; Kássio M G Lima Journal: Trends Analyt Chem Date: 2017-09-21 Impact factor: 12.296