UNLABELLED: Previous studies showed that angiotensin (Ang) II and Ang-(1-7) concentrations were reduced in the implantation site at day 7 of pregnancy in Sprague-Dawley rats as compared to the site immediately adjacent to it, which does not have the embryo attached, clearly showing the importance of the blastocyst in the regulation of renin-angiotensin system (RAS). OBJECTIVE: The objective of this study was to evaluate the regulation of the RAS in the decidualized uterus in the pseudopregnant rat, a model without the presence of a conceptus. METHODS: Ovariectomized, adult female rats were sensitized for the decidual cell reaction with steroid treatments; decidualization was induced by oil-injection of the right horn; the left horn served as a control. The uterine content of Ang I, Ang II, and Ang-(1-7) was examined in the decidualized and non-decidualized uteri. RESULTS: Both Ang-(1-7) and Ang II and ACE and ACE2 mRNA were significantly reduced in the decidualized horn as compared to the non-decidualized horn. Immunocytochemical characterization of Ang II, Ang-(1-7), ACE and ACE2 demonstrated that Ang-(1-7), Ang II, and ACE2 polarize to the anti-mesometrial pole with decidualization. CONCLUSION: The decidualization process elicits marked reduction in uterine Ang II and Ang-(1-7) content as compared to the non-decidualized horn. The differential immunocytochemical expression of Ang II and Ang-(1-7) with ACE2, but not ACE in the anti-mesometrial pole of the decidualized horn may favor the formation and action of Ang-(1-7) in the anti-mesometrial pole, an area which plays a role in triggering the decidualization process.
UNLABELLED: Previous studies showed that angiotensin (Ang) II and Ang-(1-7) concentrations were reduced in the implantation site at day 7 of pregnancy in Sprague-Dawley rats as compared to the site immediately adjacent to it, which does not have the embryo attached, clearly showing the importance of the blastocyst in the regulation of renin-angiotensin system (RAS). OBJECTIVE: The objective of this study was to evaluate the regulation of the RAS in the decidualized uterus in the pseudopregnant rat, a model without the presence of a conceptus. METHODS: Ovariectomized, adult female rats were sensitized for the decidual cell reaction with steroid treatments; decidualization was induced by oil-injection of the right horn; the left horn served as a control. The uterine content of Ang I, Ang II, and Ang-(1-7) was examined in the decidualized and non-decidualized uteri. RESULTS: Both Ang-(1-7) and Ang II and ACE and ACE2 mRNA were significantly reduced in the decidualized horn as compared to the non-decidualized horn. Immunocytochemical characterization of Ang II, Ang-(1-7), ACE and ACE2 demonstrated that Ang-(1-7), Ang II, and ACE2 polarize to the anti-mesometrial pole with decidualization. CONCLUSION: The decidualization process elicits marked reduction in uterine Ang II and Ang-(1-7) content as compared to the non-decidualized horn. The differential immunocytochemical expression of Ang II and Ang-(1-7) with ACE2, but not ACE in the anti-mesometrial pole of the decidualized horn may favor the formation and action of Ang-(1-7) in the anti-mesometrial pole, an area which plays a role in triggering the decidualization process.
Authors: Liomar A A Neves; Aleck F Williams; David B Averill; Carlos M Ferrario; Michael P Walkup; K Bridget Brosnihan Journal: Endocrinology Date: 2003-08 Impact factor: 4.736
Authors: Carlos M Ferrario; David B Averill; K Bridget Brosnihan; Mark C Chappell; Samy S Iskandar; Richard H Dean; Debra I Diz Journal: Kidney Int Date: 2002-10 Impact factor: 10.612
Authors: Michael A Crackower; Renu Sarao; Gavin Y Oudit; Chana Yagil; Ivona Kozieradzki; Sam E Scanga; Antonio J Oliveira-dos-Santos; Joan da Costa; Liyong Zhang; York Pei; James Scholey; Carlos M Ferrario; Armen S Manoukian; Mark C Chappell; Peter H Backx; Yoram Yagil; Josef M Penninger Journal: Nature Date: 2002-06-20 Impact factor: 49.962
Authors: K Bridget Brosnihan; Liomar A A Neves; JaNae Joyner; David B Averill; Mark C Chappell; Renu Sarao; Josef Penninger; Carlos M Ferrario Journal: Hypertension Date: 2003-07-21 Impact factor: 10.190
Authors: João Vaz-Silva; Rubens L Tavares; Márcia C Ferreira; Kinulpe Honorato-Sampaio; Ines K D Cavallo; Robson A S Santos; Adelina M dos Reis; Fernando M Reis Journal: J Mol Histol Date: 2012-06-09 Impact factor: 2.611
Authors: K Bridget Brosnihan; Victor M Pulgar; Patricia E Gallagher; Liomar A A Neves; Liliya M Yamaleyeva Journal: Reprod Biol Endocrinol Date: 2015-01-17 Impact factor: 5.211
Authors: K Bridget Brosnihan; Victor M Pulgar; Manish S Bharadwaj; Liomar A A Neves; Liliya M Yamaleyeva Journal: Reprod Biol Endocrinol Date: 2016-10-18 Impact factor: 5.211