L M Ruiz1, M Castro, A Barriga, C A Jerez, N Guiliani. 1. Laboratorio de Comunicaciones Bacterianas, Departamento de Biología, Facultad de Ciencias, Universidad de Chile, Santiago, Chile.
Abstract
AIMS: The primary goal of this study was to characterize the existence of a functional c-di-GMP pathway in the bioleaching bacterium Acidithiobacillus ferrooxidans. METHODS AND RESULTS: A bioinformatic search revealed that the genome sequence of At. ferrooxidans ATCC 23270 codes for several proteins involved in the c-di-GMP pathway, including diguanylate cyclases (DGC), phosphodiesterases and PilZ effector proteins. Overexpression in Escherichia coli demonstrated that four At. ferrooxidans genes code for proteins containing GGDEF/EAL domains with functional DGC activity. MS/MS analysis allowed the identification of c-di-GMP in nucleotide preparations obtained from At. ferrooxidans cells. In addition, c-di-GMP levels in cells grown on the surface of solid energetic substrates such as sulfur prills or pyrite were higher than those measured in ferrous iron planktonic cells. CONCLUSIONS: At. ferrooxidans possesses a functional c-di-GMP pathway that could play a key role in At. ferrooxidans biofilm formation during bioleaching processes. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first global study about the c-di-GMP pathway in an acidophilic bacterium of great interest for the biomining industry. It opens a new way to explore the regulation of biofilm formation by biomining micro-organisms during the bioleaching process.
AIMS: The primary goal of this study was to characterize the existence of a functional c-di-GMP pathway in the bioleaching bacterium Acidithiobacillus ferrooxidans. METHODS AND RESULTS: A bioinformatic search revealed that the genome sequence of At. ferrooxidans ATCC 23270 codes for several proteins involved in the c-di-GMP pathway, including diguanylate cyclases (DGC), phosphodiesterases and PilZ effector proteins. Overexpression in Escherichia coli demonstrated that four At. ferrooxidans genes code for proteins containing GGDEF/EAL domains with functional DGC activity. MS/MS analysis allowed the identification of c-di-GMP in nucleotide preparations obtained from At. ferrooxidans cells. In addition, c-di-GMP levels in cells grown on the surface of solid energetic substrates such as sulfur prills or pyrite were higher than those measured in ferrous iron planktonic cells. CONCLUSIONS: At. ferrooxidans possesses a functional c-di-GMP pathway that could play a key role in At. ferrooxidans biofilm formation during bioleaching processes. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first global study about the c-di-GMP pathway in an acidophilic bacterium of great interest for the biomining industry. It opens a new way to explore the regulation of biofilm formation by biomining micro-organisms during the bioleaching process.
Authors: Lara Rajeev; Eric G Luning; Sara Altenburg; Grant M Zane; Edward E K Baidoo; Michela Catena; Jay D Keasling; Judy D Wall; Matthew W Fields; Aindrila Mukhopadhyay Journal: Front Microbiol Date: 2014-07-29 Impact factor: 5.640
Authors: Stephan Christel; Malte Herold; Sören Bellenberg; Mohamed El Hajjami; Antoine Buetti-Dinh; Igor V Pivkin; Wolfgang Sand; Paul Wilmes; Ansgar Poetsch; Mark Dopson Journal: Appl Environ Microbiol Date: 2018-01-17 Impact factor: 4.792
Authors: Magnus Bosse; Alexander Heuwieser; Andreas Heinzel; Arno Lukas; Guilherme Oliveira; Bernd Mayer Journal: PLoS One Date: 2018-08-09 Impact factor: 3.240