Literature DB >> 220955

Nucleotide metabolism by microsomal UDP-glucuronyltransferase and nucleoside diphosphatase as determine by 31P nuclear-magnetic-resonance spectroscopy.

S A Finch, T F Slater, A Stier.   

Abstract

31P n.m.r. spectroscopy was used to study the nucleotide kinetics of UDP-glucuronyltransferase and associated reactions in the liver microsomal fraction. The effects of Mg2+ and EDTA on these reactions were investigated qualitatively. It was found that the rabbit microsomal fraction has no nucleoside pyrophosphatase activity, that UDP was immediately hydrolysed and that it was released from the microsomal surface. Reverse glucuronyltransferase could be demonstrated. The results are discussed with reference to functional coupling of UDP-glucuronyltransferase to other enzymes and the effects of Mg2+ and EDTA on the system.

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Year:  1979        PMID： 220955      PMCID： PMC1186459          DOI： 10.1042/bj1770925

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  7 in total

1.  The effect of phospholipase C treatment on the latency and sedimentability of rat liver microsomal nucleoside diphosphatase.

Authors:  K O'Toole
Journal:  FEBS Lett       Date:  1975-04-01       Impact factor: 4.124

2.  Heterogeneous distribution of glucose 6-phosphatase in rat liver microsomal fractions as shown by adaptation of a cytochemical technique.

Authors:  J A Lewis; J R Tata
Journal:  Biochem J       Date:  1973-05       Impact factor: 3.857

3.  Studies on microsomal nucleoside diphosphatase of rat hepatocytes. Its purification, intramembranous localization, and turnover.

Authors:  Y Kuriyama
Journal:  J Biol Chem       Date:  1972-05-25       Impact factor: 5.157

4.  Summary of a novel, three-component regulatory model for uridine diphosphate glucuronyltransferase.

Authors:  C Berry; T Hallinan
Journal:  Biochem Soc Trans       Date:  1976       Impact factor: 5.407

5.  Non-lamellar structure in rabbit liver microsomal membranes: a 31P-NMR study.

Authors:  A Stier; S A Finch; B Bösterling
Journal:  FEBS Lett       Date:  1978-07-01       Impact factor: 4.124

6.  A rapid NAD+-linked assay for microsomal uridine diphosphate glucuronyltransferase of rat liver and some observations on substrate specificity of the enzyme.

Authors:  G J Mulder; A B van Doorn
Journal:  Biochem J       Date:  1975-10       Impact factor: 3.857

7.  Permeability of microsomal membranes isolated from rat liver.

Authors:  R Nilsson; E Peterson; G Dallner
Journal:  J Cell Biol       Date:  1973-03       Impact factor: 10.539
  7 in total
  2 in total

1.  Carrier-mediated translocation of uridine diphosphate glucose into the lumen of endoplasmic reticulum-derived vesicles from rat liver.

Authors:  F Vanstapel; N Blanckaert
Journal:  J Clin Invest       Date:  1988-09       Impact factor: 14.808

2.  Further validation of the Mulder and van Doorn kinetic procedure for the measurement of microsomal UDP-glucuronosyltransferase activities.

Authors:  B Antoine; J A Boutin; G Siest
Journal:  Biochem J       Date:  1988-06-15       Impact factor: 3.857
  2 in total

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