Literature DB >> 22094814

Ribosome display for the selection of Sac7d scaffolds.

Barbara Mouratou1, Ghislaine Béhar, Lauranne Paillard-Laurance, Stéphane Colinet, Frédéric Pecorari.   

Abstract

Combinatorial libraries of Sac7d have proved to be a valuable source of proteins with favorable biophysical properties and novel ligand specificities, so-called Nanofitins. Thus, Sac7d represents a promising scaffold alternative to antibodies for biotechnological and potentially clinical applications. We describe here the methodology for the construction of a library of Sac7d and its use for selection by ribosome display.

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Year:  2012        PMID: 22094814     DOI: 10.1007/978-1-61779-379-0_18

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN: 1064-3745


  5 in total

Review 1.  Beyond Antibodies as Binding Partners: The Role of Antibody Mimetics in Bioanalysis.

Authors:  Xiaowen Yu; Yu-Ping Yang; Emre Dikici; Sapna K Deo; Sylvia Daunert
Journal:  Annu Rev Anal Chem (Palo Alto Calif)       Date:  2017-03-24       Impact factor: 10.745

2.  The archaeal "7 kDa DNA-binding" proteins: extended characterization of an old gifted family.

Authors:  Valentina Kalichuk; Ghislaine Béhar; Axelle Renodon-Cornière; Georgi Danovski; Gonzalo Obal; Jacques Barbet; Barbara Mouratou; Frédéric Pecorari
Journal:  Sci Rep       Date:  2016-11-17       Impact factor: 4.379

3.  Potent and specific inhibition of glycosidases by small artificial binding proteins (affitins).

Authors:  Agustín Correa; Sabino Pacheco; Ariel E Mechaly; Gonzalo Obal; Ghislaine Béhar; Barbara Mouratou; Pablo Oppezzo; Pedro M Alzari; Frédéric Pecorari
Journal:  PLoS One       Date:  2014-05-13       Impact factor: 3.240

4.  Use of the Nanofitin Alternative Scaffold as a GFP-Ready Fusion Tag.

Authors:  Simon Huet; Harmony Gorre; Anaëlle Perrocheau; Justine Picot; Mathieu Cinier
Journal:  PLoS One       Date:  2015-11-05       Impact factor: 3.240

5.  Discovery of APL-1030, a Novel, High-Affinity Nanofitin Inhibitor of C3-Mediated Complement Activation.

Authors:  Joshua Garlich; Mathieu Cinier; Anne Chevrel; Anaëlle Perrocheau; David J Eyerman; Mark Orme; Olivier Kitten; Lukas Scheibler
Journal:  Biomolecules       Date:  2022-03-11
  5 in total

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